The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
It may be desirable to know how such small differences as those betwixt
the various thin glass covers can be measured. For this purpose there
are two or three sorts of apparatus, all, however, depending upon the
same principle. The description of one, therefore, will be sufficient.
Upon a small stand is a short metal _lever_ (as it may be termed) which
returns by a spring to one certain position, where it is in contact
with a fixed piece of metal. At the other end this lever is connected
with a “finger,” which moves round a dial like that of a watch,
whereupon are figures at fixed distances. When the lever is separated
from the metal which is stationary, the other end being connected
with the “finger” of the dial, that “finger” is moved in proportion
to the distance of the separation. The thin glass is, therefore,
thrust betwixt the end of the lever and fixed metal, and each piece is
measured by the figures on the dial in stated and accurate degrees.
This kind of apparatus, however, is expensive, and when not at our
command, the thin glass may be placed edgewise in the stage forceps,
and measured very accurately with the micrometer.
Cleanliness with the thin glass is, perhaps, more necessary than with
the slides, especially when covering objects which are to be used with
a high power; but it is far more difficult to attain, on account of
the liability to breakage. The usual method of cleaning these covers
is as follows:--Two discs of wood, about two inches in diameter, are
procured, one side of each being perfectly flat and covered with clean
wash-leather. To the other side of these a small knob is firmly affixed
as a handle, or where practicable the whole may be made out of a solid
piece. In cleaning thin glass, it should be placed betwixt the covered
sides of the discs, and may then be safely rubbed with a sufficient
pressure, and so cleaned on both sides by the leather. If, however,
the glass be greasy, as is sometimes the case, it must be first washed
with a strong solution of potash, infusion of nut-galls, or any of
the commonly used grease-removing liquids; and with _some_ impurities
water, with the addition of a few drops of strong acid, will be found
very useful, but this last is not often required.
The above method of cleaning thin glass should always be used by
beginners; but after some experience the hand becomes so sensitive
that the above apparatus is often dispensed with, and the glasses,
however thin, may be safely cleaned betwixt the fingers and thumb with
a cambric handkerchief, having first slightly damped the ends of the
fingers employed to obtain firm hold. When the dirt is very obstinate,
breathing upon the glass greatly facilitates its removal, and the sense
of touch becomes so delicate that the breakage is inconsiderable; but
this method cannot be recommended to novices, as nothing but time spent
in delicate manipulation can give the sensitiveness required.
Public-domain text, read in full here on John Shaqi.
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