The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Mr. T. G. Rylands’ method differs in some degree from the above, and
is, to use his own words, as follows:--Thick balsam is preferable,
and the burnt covers (see Chapter II.) to be mounted are laid in a
convenient position with the diatoms upwards. The slides required
having been carefully cleaned and marked on the under side with a ring
of ink, by the aid of a turntable about half an inch in diameter to
point out the centre, a drop of benzole is applied by a large pin to
the diatoms on the cover, so as to exclude the air from the valves
and frustules. The slide is then held over the lamp, and when warm,
a sufficiently large drop of balsam is put upon it, and heated until
it begins to steam. If small bubbles appear, a puff of breath removes
them. The slide being held slightly inclined from the operator, and the
drop of balsam becoming convex at its lower edge, the cover is brought
in contact with it at that point, gradually laid down, pressed with the
forceps, and brought to its central position. When cool the superfluous
balsam (if any) is removed with a heated knife-blade, the slide cleaned
with a little turpentine, and finished by washing in a hand-basin with
soap and water. In this process there is no delay if the balsam be
sufficiently thick, as the slide may be cleaned off almost before it is
cold.
It is now well known that from common chalk it is an easy matter to
obtain interesting specimens of Foraminifera. Scrape a small quantity
of chalk from the mass and shake it in water; leave this a few minutes,
pour the water away and add a fresh quantity, shake up as before, and
repeat two or three times. Take a little of the residue, and spread it
upon the slide, and when quite dry add a little turpentine. When viewed
with a power of two hundred and fifty diameters this will generally
show the organisms very well. If it is desired to preserve the slides,
they may be then mounted in Canada balsam. Mr. Guyon, in “Recreative
Science,” observes that the accumulation of the powder, by the action
of the rain or exposure to the atmospheric action, at the foot or any
projection of the chalk cliffs, will afford us better specimens than
that which is “scraped,” as the organisms are less broken in the former.
When the Foraminifera are of a larger size, though transparent enough
to be mounted in balsam, the air must be first expelled from the
interior, otherwise the objects will be altogether unsatisfactory. To
accomplish this they must be immersed in turpentine and submitted to
the action of the air-pump. So difficult is it to get rid of this enemy
that it is often necessary to employ three or four exhaustions, leaving
them for some time under each. When all air has given place to the
turpentine, they must be mounted in the ordinary way.
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