The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Sections of some exquisitely beautiful objects are cut with much less
trouble than the above. The Orbitolite, for instance, may be prepared
in this manner. Take the object and by pressure with the finger rub
the side upon a flat and smooth sharpening stone with water until the
portion is reached which it is wished to show. The strength of the
object will easily allow this to be accomplished with ordinary care.
This side may then be attached to the glass slide with heated balsam,
as above described, and the object may then be gently rubbed down to
the degree of thinness required to show it to the best advantage.
After removing all disengaged matter from the object by washing and
thoroughly drying, it may be mounted in balsam in the usual manner,
when it is equally beautiful as a transparent or opaque object. From
this it will be seen that in many instances where a smooth stone is
found sufficient for the work (which is often the case when the section
is mounted in balsam) the final process of polishing advised above
may be dispensed with, as in the Orbitolite, Nummulite, &c., &c. It
is quite necessary that the stones on which the objects are rubbed
be _perfectly flat_, otherwise one side must be acted upon before
the other, and it will be found impossible to attain anything like
uniformity. Where it is not practicable to cut a section and the object
is very thick, a coarse stone may be first used to reduce it and the
smoother afterwards.
The consideration of the cutting of sections from shells would scarcely
be deemed complete without some mention of what Dr. Carpenter terms the
decalcifying process. Muriatic acid is diluted with twenty times its
volume of water, and in this the shell is immersed. After a period,
differing according to the thickness of the shell, the carbonate of
lime will be dissolved away, and a peculiar membrane left, showing
the structure of the shell very perfectly. This may be mounted dry,
in balsam, or sometimes in liquid, according to the appearance of
the object; but no rule can be given. The discretion of the student,
however, will enable him to choose the most suitable method.
From some shells it is easy to divide thin plates, or “laminæ,” which
require nothing but mounting in Canada balsam to show the texture very
well. In working, however, with those which are “pearly,” it will
be found that experience and patience are needed, as they are very
brittle and peculiarly hard; but a little practice will overcome these
difficulties.
Public-domain text, read in full here on John Shaqi.
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