The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Perhaps the best method which can be pursued is that recommended in the
“Micrographic Dictionary,” which is as follows:--“The coal is macerated
for about a week in a solution of carbonate of potash; at the end of
that time it is possible to cut tolerably thin slices with a razor.
These slices are then placed in a watch-glass with strong nitric acid,
covered and gently heated; they soon turn brownish, then yellow, when
the process must be arrested by dropping the whole into a saucer of
cold water, else the coal would be dissolved. The slices thus treated
appear of a darkish amber colour, very transparent, and exhibit the
structure, when existing, most clearly. We have obtained longitudinal
and transverse sections of coniferous wood from various coals in this
way. The specimens are best preserved in _glycerine_ in cells; we find
that spirit renders them opaque, and even Canada balsam has the same
defect. Schulz states that he has brought out the cellulose reaction
with iodine, in coal treated with nitric acid and chlorate of potash.”
_Cannel-coal_ is so close and firm in its structure as to be much used
instead of jet in the manufacture of ornaments: it takes a beautiful
polish, and consequently presents the student with none but ordinary
difficulties in getting sections of it. Its formation is somewhat
different from that of coal, sometimes showing the transition very
clearly.
In _flint_ there are often found remains of sponges, shells,
Diatomaceæ, &c.; but to show these well, sections must be cut and
polished by the lathe and wheel of the lapidary, which the microscopic
student seldom possesses. Thin chippings may, however, be made, which
when steeped in turpentine and mounted in balsam, will frequently show
these remains very well.
_Teeth_ are very interesting objects to all microscopists, more
especially to those who give much study to them; as the class of animal
may very frequently be known from one solitary remaining tooth. To
examine them thoroughly, it is necessary to cut sections of them; but
this is rather difficult to perform well, and needs some experience.
Some instructions, however, will at least lessen these difficulties,
and we will now endeavour to give them.
Public-domain text, read in full here on John Shaqi.
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