The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
An interesting object may also be procured from whalebone by cutting
long sections of the hairs of which it is composed. Down the centre
of each hair we shall find a line of cells divided from one another
very distinctly. And (as recommended in the “Micrographic Dictionary”)
if whalebone be macerated twenty-four hours in a solution of caustic
potash it will be softened, and by afterwards digesting in water, the
outer part will be resolved into numerous transparent cells, which will
show more plainly the structure of this curious substance.
In a former chapter, hairs were mentioned, their many and interesting
forms, and their beauty when used with polarized light. The sections of
them, however, are no less a matter of study, as this mode of treatment
opens to sight the outer “casing,” and the inner substance somewhat
resembling the pith of plants.
It would be out of place to enter into the description of the different
forms met with; but the ways in which sections are to be procured
may be glanced at. If transverse sections are required, some place a
quantity of hairs betwixt two flat pieces of cork, which by pressure
hold them firmly enough together to allow the required portions to be
cut with a razor. Others take a bundle of the hairs and dip it into gum
or glue, which gives it when dry a solidity equal to wood. Sections of
this are then cut with the machine mentioned a little further on, and
these may be mounted in balsam. The human hair is easily procured in
the desired sections by shaving as closely as possible a second time
and cleansing from the lather, &c., by carefully washing. Most hairs,
however, should be examined both transversely and longitudinally. It
is not difficult to procure the latter, as we may generally split them
with the aid of a sharp razor. In a great number of hairs there is a
quantity of greasy matter which must be got rid of by soaking in ether
or some other solvent before mounting.
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