The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
We may next consider the best method of procuring _sections of wood_,
which must be cut of such a degree of thinness as to form transparent
objects, and so display all the secrets of their structure. There
is no monotony in this study, as the forms are so various, and the
arrangement of the cells and woody fibre so different, that the
microscopist may find endless amusement or study in it. From a single
section the _class_ of trees to which it has belonged may be known,
often even when the wood is _fossil_. The apparatus best adapted for
cutting these sections is made as follows:--A flat piece of hard wood,
about six inches long, four wide, and one thick, is chosen, to which
another of the same size is firmly fixed, so as to form, in a side
view, the letter =T=. On one end of the upper surface is fastened a
brass plate, perfectly flat, in the centre of which a circular opening
is cut about half an inch in diameter. Coinciding with this opening
is a brass tube, fixed in the under side of the table (if it may be
termed so). This tube is so cut at the bottom as to take a fine screw.
Another screw is also placed at the same end of the “table,” which
works at right angles to this, so that any substance in the tube may be
wedged firmly by working this last screw. To use this instrument, the
piece of wood or other object of which a section is required must be
placed in the tube, when, by turning the screw underneath, the wood is
raised above the brass plate more or less as wished, and by using the
screw at the end, it is held firmly in the same position. With a flat
chisel the portion of the object which projects above the surface of
the brass plate may now be cut off, and by means of the bottom screw
another portion may be raised and treated in the same manner. As to
the thickness of which objects should be cut, no proper directions can
be given, as this differs so greatly that nothing but experience can
be any guide. The same thickness can be obtained by working the screw
underneath in uniform degrees, the head being marked for this purpose;
and when the substance to be cut is _very_ much smaller than the hole
in the brass plate, it may be wedged with cork.
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