The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
As this instrument is peculiarly adapted for cutting wood (though
used for other substances, as before mentioned), I shall notice a
few particulars concerning this branch of sections. It may here be
remarked, that to obtain anything like a true knowledge of the nature
of wood, it should be cut and examined in at least two directions,
_across_ and _along_. The piece of wood is often placed in spirits
for a day or two, so that all resinous matter may be dissolved out of
it; it must then be soaked in water for the same length of time, so
as to soften and render it easy to cut. Sections are obtained in the
manner described above, but often curl to such a degree as to make it
necessary to immerse them in water, from which they may be taken and
dried under slight pressure. They are often mounted _dry_, and require
no care beyond other objects, as in Chapter II. Some, however, are
best mounted in balsam, particularly the long sections when used for
the polariscope; these must be soaked in turpentine, and the greatest
care taken that all air bubbles are got rid of. Others are thought
to be most useful when mounted in shallow cells with some of the
_preservative liquids_ mentioned in Chapter IV.--weak spirit and water,
chloride of calcium solution of the strength of one part of the salt to
three parts of distilled water, &c.
The above “_section-cutter_” may not be within the reach of every
student, nor is it absolutely necessary; though where any _great
number_ of specimens is required it is very useful, and insures greater
uniformity in the thickness. Many employ a razor for the purpose, which
must always be kept sharp by frequent stropping. Sections of leaves
also may be procured by the same means, though, as before mentioned,
they are sometimes easily divided by stripping the coatings off with
the fingers. The cells which come to sight by cutting some of the
orchideous plants are most interesting. To cut these leaves they
may be laid upon a flat piece of cork, thus exposing the razor to no
danger of injury by coming in contact with the support. It may be
mentioned here that the _razor_ may also be used in cutting sections
of the rush, than which a more beautiful object can scarcely be found
when viewed transversely, as it shows the stellate arrangements of the
ducts to convey the liquids to the different parts of the plant very
clearly. This should be mounted _dry_. In the same way sections of the
leaf-stalks of ferns may also be cut, some of which, as Dr. Carpenter
states, show the curious ducts very beautifully, especially when cut
rather obliquely.
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