The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
There are many who almost confine their attention to polarized light
and its beautiful effects. Such would not deem these efforts to aid
the student in cutting sections complete without some notice of those
which are taken from various crystals, in order to display that curious
and beautiful phenomenon, _the rings with a cross_. The arrangement of
these is somewhat changed by the crystal which affords the section; but
nitrate of potash gives two sets of rings with a cross, the long line
of which passes through both, the short line dividing it in the middle.
The process of cutting these sections is rather difficult, but a
little care and perseverance will conquer all this. The following is
extracted from the _Encyclopædia Metropolitana_:--“Nitre crystallizes
in long six-sided prisms whose section, perpendicular to their sides,
is the regular hexagon. They are generally very much interrupted in
their structure; but by turning over a considerable quantity of the
ordinary saltpetre[F] of the shops specimens are readily found which
have perfectly transparent portions of some extent. Selecting one
of these, cut it with a knife into a plate _above_ a quarter of an
inch thick, directly across the axis of the prism, and then grind it
down on a broad wet file till it is reduced to about one quarter or a
sixth of an inch thick, smooth the surface on a wet piece of emeried
glass, and polish on a piece of silk strained very tight over a strip
of plate-glass, and rubbed with a mixture of tallow and colcothar
of vitriol. This operation requires practice. It cannot be effected
unless the nitre be applied wet and rubbed till quite dry, increasing
the rapidity of the friction as the moisture evaporates. It must be
performed in gloves, as the vapour from the fingers, as well as the
slightest breath, dims the polished surface effectually. With these
precautions a perfect vitreous polish is easily obtained. We may here
remark, that hardly any two salts can be polished by the same process.
Thus, Rochelle-salt must be finished wet on the silk, and instantly
transferred to soft bibulous linen and rapidly rubbed dry. Experience
alone can teach these peculiarities, and it is necessary to resort to
contrivances (sometimes very strange ones) for the purpose of obtaining
good polished sections of soft crystals, especially of those easily
soluble in water.
“The nitre is thus polished on both its surfaces, which should be
brought as near as possible to parallelism.”
[F] Sometimes the saltpetre of the shops is nitrate of _soda_,
and as this is slightly deliquescent, it is well to be
certain that we have the nitrate of _potash_, which is free
from this defect.
Public-domain text, read in full here on John Shaqi.
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