The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Some sections of the naturally formed crystals also show the “rings”
very well,--as Iceland Spar, which gives a single ring and cross; but
the difficulty of cutting and polishing them is almost too great for
the amateur, and must be left to the lapidary. This curious phenomenon,
however, may be seen by using a plate of ice uninterruptedly formed of
about one inch in thickness.
Before concluding these remarks on sections, I must mention a few
difficulties which may be met with, and their remedies. The foremost of
these is the softness of some objects, which have not resistance enough
in themselves to bear cutting even with the sharpest instruments.
This may often be removed by soaking in a solution of gum, and then
drying, which will render the substance firm enough to be cut, when the
sections must be steeped in water, and the gum thus got rid of. Small
seeds, &c., may be placed in wax when warmed, and will be held firmly
enough when it is again cold to allow of them being cut into sections,
&c. And, lastly, where a substitute for a microscopist’s hand-vice is
required, a cork which fits any tube large enough may be taken and
split, the object being then placed between the two parts, and the cork
thrust into the tube, a sufficient degree of firmness will be obtained
to resist any necessary cutting, &c.
DISSECTION.--As I stated at the commencement of this chapter, no
written instructions can enable any student to become an adept in this
branch without much experience and no little study. I will, however,
describe the necessary apparatus, and afterwards mention the mode of
treatment which certain objects require.
A different microscope is manufactured for the purpose of dissection,
most first-rate makers having their own model. The object-glasses of
many of these are simple, and consequently not expensive; but one of
the great requisites is a stage large enough to hold the trough, in
which the operation is often performed. Where this is the case it would
scarcely be worth the expense of getting a dissecting microscope if the
student were pursuing no particular study, but merely made use of the
instrument when an object to be operated upon turned up accidentally.
The ordinary form is much improved for this purpose, by having two
wooden rests placed at the sides of the microscope, upon which the
hands may be supported when working upon the stage. These should be
weighty enough to be free from danger of moving. These supports will
also be found to be a remedy against much of the weariness which
inevitably arises from having to sustain the hands as well as work
with them. The erector, as I before observed, is necessary to a young
student; but with a little practice he may work very well without it.
Public-domain text, read in full here on John Shaqi.
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