The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
“But the peculiarity of the toothed membrane, which makes its name of
‘_ribbon_’ so appropriate, is, that there is always a considerable
length of it behind the mouth, perfectly formed, and ready to come
forward and supply the place of that at the front, which is continually
wearing away by use.
“In the limpet this reserve-ribbon is of great length, being nearly
twice as long as the body, and the whole of it is exposed to view on
simply removing the foot of the animal; nothing, then, can be easier
than to extract the tongue of the common limpet. But, unfortunately,
what you find in one kind of mollusc is not at all what you find in
another. In the Acmæas, for instance, which are very closely related
to the limpets, and have shells which cannot be distinguished, the
reserve portion of the ribbon has to be dug out from the substance
of the liver, in which it is imbedded, that organ being, as it were,
stitched completely through by a long loop of it.... It might be
thought a comfortable reflection that, at all events, one end of the
ribbon can always be found in the mouth; but in many cases this is
about the worst place to look for it. Perhaps it may appear strange
that in some of the smaller species, with a retractile trunk, a
beginner may very likely fail altogether in his attempt to find the
mouth; if, however, the skin of the back is removed, commencing just
behind the tentacles, there will be very little difficulty in making
out the trunk, which either contains the whole of the ribbon, as in
the whelk, or the front part of it, as in _Purpura_ and _Murex_, where
a free coil is also seen to hang from its hinder extremity.... In the
periwinkles the same plan of proceeding, by at once opening the back of
the animal, is best; and on doing so, the long ribbon, coiled up like a
watch-spring, cannot fail to be found.
“In the Trochuses, and indeed in all the _Scutibranchiata_, one point
of the scissors should be introduced into the mouth of the animal, and
an incision made directly backwards in the middle line above to some
distance behind the tentacles; the tongue is then immediately brought
into view, lying along the floor of the mouth.”
Dr. Alcock’s method of dissection will be found to differ in some
degree from the general rules before given; and when the tongue is
dissected out he washes it for one hour (shaking it now and then) in a
weak solution of potash. After cleaning thoroughly in water, it must be
mounted by one of the methods before mentioned.
Amongst insects, especially the grasshopper tribe, are found many which
possess a gizzard, armed with strong teeth, somewhat similar to those
of the molluscs. It requires great nicety of manipulation to obtain
these for the microscope; but it would be useless to attempt any
description of the process here, as the strident can only be successful
by experience in dissecting objects less difficult to manage, and by
using the most delicate instruments.
Public-domain text, read in full here on John Shaqi.
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