The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
“This closes my present communication on the tongues of mollusca; but
as some members may possibly feel inclined to enter upon the inquiry
themselves, I think it will not be amiss to add a few remarks on the
manner in which they are to be obtained.
“First, as to the kinds best worth the trouble of preparation. Whelks,
Limpets, and Trochuses should be taken first. Land and fresh-water
snails can scarcely be recommended, except as a special study,--their
tongues being rather more difficult to find, and the teeth so small
that they require a high power to show them properly. It would appear,
from Spallanzani’s description of the anatomy of the head of the snail,
that even he did not make out this part, although, in his curious
observations on the reproduction of lost parts, he must have carefully
dissected more snails than any other man.
“As to preserving the animals till wanted, they should simply be
dropped alive into glycerine or alcohol. Glycerine is perhaps best
where only the tongues are wanted; but it leaves the animals very soft;
and as it does not harden their mucus at all, they are very slippery
and difficult to work upon when so preserved.
“Then as to the apparatus required for dissection. In the first
place, all the work is to be done under water, and a common saucer is
generally the most convenient vessel to use. No kind of fastening down
or pinning out of the animal is needed; and, in fact, it is much better
to have it quite free, that you may turn it about any way you wish.
The necessary instruments are a needle-point, a pair of fine-pointed
scissors, and small forceps; the forceps should have their points
slightly turned in towards each other.
“A word or two on the lingual apparatus generally, and on its special
characters in a few different animals, will conclude what I have to say.
“The mode of using the tongue can be easily seen in any of the common
water-snails, when they are crawling on the glass sides of an aquarium;
it may then be observed that from between the fleshy lips a thick
mass is protruded, with a motion forwards and upwards, and afterwards
withdrawn, these movements being almost continually repeated. The
action has the appearance of licking; but when the light falls suitably
on the protruded structure, it is seen to be armed with a number of
bright points, which are the lingual teeth, so arranged as to give the
organ the character and action of a rasp.
“If you proceed to dissection, and open the head of one of these
mollusca (say, for instance, a common limpet), you will find the cavity
of the mouth almost filled with the thick fleshy mass, the front of
which is protruded in the act of feeding; and on its upper surface,
extending along the middle line from back to front, is seen the strong
membranous band upon which the teeth are set. The mass itself consists
of a cartilaginous frame, surrounded by strong muscles; and these
structures constitute the whole of the active part of the lingual
apparatus....
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