The controversy was conducted by Dr. Roberts against Dr. Bastian, while all the experiments of Professor Tyndall were converging upon the same conclusions as those reached by Roberts.[L] That Bastian had obtained bacteria after boiling, admitted of no doubt, and he naturally clung to this fact as encouraging; others regarded it as only misleading. Bastian maintained that the alkali had a positive power of originating life, and stated one hundred and twenty-two degrees Fahr. as favorable to the appearance of life. Roberts took ten examples of sterilized urine, and twenty-nine examples of fermentible liquids which had remained over from the earlier experiments of 1873-74, and these thirty-nine examples were subjected to careful experiment and observation. In the first ten cases, the tube was heated in oil for fifteen minutes up to two hundred and eighty degrees Fahr.; the ten tubes were then set in a warm place (from seventy degrees to eighty degrees Fahr.) for a fortnight; the contents were transparent; the alkali was then allowed to mingle with it, and the tubes were placed in an incubator kept at a temperature of one hundred and fifteen degrees Fahr.; at the end of two days there was a sediment, and the liquor was clear; the tubes were replaced in the incubator, the temperature being raised to one hundred and twenty-two degrees Fahr. as recommended by Dr. Bastian; there they continued for three days; they were then withdrawn and placed under the microscope, but no trace of living organism was found either in the fluid or in the deposit under it. The twenty-nine cases, including a variety of vegetable and animal preparations were next treated in like manner, and with like results. Tyndall's experiments were reported to the Royal Society of London at the same time, with exactly the same result. M. Pasteur had previously reported to the Academy of Sciences in Paris to the same effect. It was thus proved by a mass of evidence that if proper precautions were taken to destroy germinal forms, no mixture of alkali with acid, whatever the variety of materials selected, was adequate to produce life.