The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
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The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
For section cutting, special razors are sold, they are heavy as a rule
and not hollow ground. Many microscopists aver that good sections
cannot be cut with a hollow ground razor, we beg to differ, however.
On this point we would advise our readers to select whichever pattern
suits them best; there is no need to buy an expensive razor, for our
early efforts, at anyrate, will quickly dull the cutting edge and
there is no advantage in going to much expense in this direction.
To cut really good sections is not difficult, it is a question of knack
rather than skill; having once acquired the knack, practice will do the
rest. The great point is to begin properly, some people never take the
necessary trouble in their early days and, as a consequence, they never
learn to cut good sections.
There is nothing mysterious about the operation, a section is merely a
very thin slice. A good section for examination under the microscope
should, of course, be so thin that it is transparent; it should be
equally thin everywhere, not thick in some places and thin in others
and it should be cut in the proper direction. The last statement
requires a little explanation; suppose we wish to make a section
straight across a stem, a transverse section it would be called, then
we must see to it that our section is cut straight across, and not in
such a manner that the portion from which the section is cut tapers
in the slightest degree. Before we begin the actual section cutting
we must always trim up our specimen with one of the dissecting knives
and not attempt to make the surface level with the razor. As a start,
let us take some moderately fleshy plant stem for we shall find it as
easy to cut as anything. Our stem must not be woody, for till we have
had a fair amount of practice we shall damage our razor on its hard
structures; it must not be too soft or it will be crushed out of all
recognition in our fingers; it should not be hollow, for solid stems
are always easier to cut. Having selected our stem and the spot upon it
at which we require a section, we cut straight across it at that spot
with our dissecting knife, in such a manner that the cut edge is as
nearly as possible at right angles to the sides of the stem. A piece
two or three inches long is the most comfortable to hold.
Public-domain text, read in full here on John Shaqi.
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