The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
History
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
Having cut a section it is hardly likely that, at this our first
attempt, it will be good enough to put under the microscope, so we
will continue to cut section after section till we have a dozen or
more from these we can select the most transparent for examination.
The sections of such material as a stem should be kept moist and
to do so we will place them in a watch glass containing water. It is
often easier, also, to cut our sections if the razor be moistened with
water, at anyrate the moisture prevents the sections from adhering to
the razor. The sections should be removed from razor to watch glass
and from watch glass to slide, by means of a brush, never by means of
the fingers. The razor of course, should be well dried with a soft rag
before it is put away; rust, besides being unsightly, ruins the cutting
edge. If we are really anxious to cut our own sections, and every good
microscopist does so, we shall return to the operation again and again,
even cutting objects which we have no desire to examine, for the sake
of the practice. We shall soon reach a stage where our razor will be
dulled and require stropping and the efficient stropping of a razor is,
to many people, a more difficult operation than the cutting of sections.
We may point out here, that all sections are not quite so easy to cut
as the one we have taken as our example. Some objects are so soft that
they need hardening with chemicals before they can be cut, some are
so hard that to attempt to cut them would ruin the edge of the razor,
though it is wonderful what hard substances may be cut when we have
had a little experience; some are so delicate that they must needs be
buried in melted wax, then object and wax are cut together and later
the wax is separated from the section.
Leaves and very small stems may be sectioned by the beginner, as
easily as larger stems. For such objects little sticks of pith are
sold as holders. Having cut of our piece of leaf from which we wish to
derive a section, we make a slit, with our dissecting knife, down the
middle of one end of a piece of pith. The piece of leaf is then placed
in the slit and by holding the pith at the sides our piece of leaf is
held firmly. Sections are cut through pith and leaf, and the two are
floated in water, when the thin slices of pith will float away from the
leaf sections.
Public-domain text, read in full here on John Shaqi.
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