The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
History
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
Having cut a satisfactory section, let us proceed to describe the
method of making a slide thereof. We will suppose that we do not
wish to make a permanent preparation but one for temporary use. A
clean slide must be selected, all through our pages we emphasise the
cleanliness of slides, at or near its centre we put a drop of water
and, lifting it with a brush, we place our section in the drop of
water. If our examination is to be with a low magnification, we need
not use a cover slip, nevertheless it is worth while to cultivate the
habit of using one. The cover slip is not only a protection for our
object but for our objective. Water you may argue cannot harm the
lenses of the objective. Perhaps not, we will not argue the point but,
when the water dries on the objective, it leaves a certain amount of
deposit on the glass and this deposit must be rubbed off. The less
often the lenses are rubbed the better for them, glass especially very
highly polished optical glass, is far more easily scratched than many
people imagine and a scratched lens is an inefficient lens. When using
high magnifications a cover glass should always cover our object and
the same remark applies to objects examined in Canada Balsam. This
substance is likely to cause serious trouble if it finds its way on to
an objective. It must be removed, that is obvious, but it sets hard,
it must not be scraped away for fear of damaging the optical glass
and, as it is used to cement the lenses together, there is the great
danger that any solvent used to remove the Balsam from the face of the
objectives, may also dissolve their setting. Our digression may seem
somewhat unnecessary, but the very great importance of keeping all
chemicals and even water, from coming into contact with the lenses of
our instrument cannot be insisted upon too strongly.
In whatever substance we examine our object, water, glycerine or
Balsam, there is a right and a wrong way of applying the cover slip. It
must not be dropped or laid down flat upon the object, if we do this
we shall certainly imprison a number of air bubbles and that must be
avoided. One edge of the cover slip must be laid against the edge of
the mountant, as the liquid used for mounting our object is called,
then placing a needle beneath the cover slip it must be gently lowered
into position. We shall now find that all air bubbles are driven out as
the cover slip is lowered.
Public-domain text, read in full here on John Shaqi.
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