The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
History
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
When we have cut an exceptionally good section or when we have some
specially interesting object we may wish to make a permanent slide.
The exact method of doing so varies somewhat with the nature of our
object and to describe all the methods of mounting microscopic objects
permanently would require a book in itself. We will suppose that we
wish to make a permanent slide of one of the sections floating in
our watch glass of water. The first thing we must do is to get rid
of the water with which our section by now is saturated. This may be
accomplished by means of alcohol of various strengths, which we may
put into three watch glasses. In the first watch glass we have half
pure alcohol and half water; in the second three-quarters alcohol and
one part water; in the third watch glass pure or absolute alcohol. The
section is transferred on a brush to the first watch glass and left
there for five minutes, then to the second watch glass for a similar
time and finally to the third watch glass. The mountant may be either
Canada Balsam or glycerine jelly. Should we decide on Canada Balsam,
we put a small drop of the substance in the centre of a clean slide,
place one section atop of it and then gently lower a cover slip upon
it as already described. Then, without using any force, the cover slip
is pressed down, but we must not fall into the all too common error of
thinking that a thick section can be made thin by pressing upon the
cover slip. If we have taken the correct amount of Canada Balsam it
will just cover the area below the cover slip and no more, if we have
used too much, it will flow out on all sides and perhaps on the top of
the cover slip. A very little experience will teach how big a drop of
Balsam to use.
Should we decide to use glycerine jelly, we take a small globule of
the jelly on one of our mounted needles and place it in the centre of
a clean slide. Then we hold the slide a little above a lighted candle,
or even a match will do, to melt the jelly. Directly it is melted
the section is placed upon it, and we proceed as before. Whichever
mountant we have used, the slides must be put away out of the dust, and
they must be flat and not placed on edge. In a few days the mountant
will set, and we need take no further precautions with the slides in
which Balsam was used, but those mounted in glycerine jelly must be
ringed, for the reason that glycerine absorbs moisture from the air and
gradually liquifies.
Public-domain text, read in full here on John Shaqi.
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