The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
History
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
We have already mentioned that the optical parts of the compound
microscope are of greater importance than what may be termed the
mechanical portions and the objectives are more important than
the eyepieces. Better results can always be obtained with a good,
high-power objective and a low-power eyepiece, than with an inferior
objective and a good quality eyepiece. The merits of the eyepiece,
however great, will not be adequate compensation for the failings of
the objective. Modern objectives are composed of several lenses and of
a combination of flint and crown glass, as we explained in our last
chapter. They are so designed that they can be screwed into the lower
part of the microscope tube. The focal length of each objective is,
or should be, marked upon it; as a general rule, however, it may be
taken that the smaller the lower lens, the shorter its focal length and
therefore the greater its magnifying power.
The form of eyepiece most usually met with is known as Huyghen’s.
It consists of two plano-convex lenses, with their flat or plane
surfaces directed away from the objective. The smaller of the two
lenses is situated nearer to the eye of the observer and is known
as the eyeglass; its function is to magnify the image formed by
the objective. The larger, lower lens is known as the field or
collecting glass; it renders the image clearer though, in so doing,
it reduces the magnification of the eyeglass. In instruments provided
with more than one eyepiece we shall wish to know which gives the
greater magnification; this is or should be marked upon the metal rim
surrounding the eyeglass but, in general, it may be stated that the
shorter the eyepiece the greater its magnification. We repeat again,
increase your magnification always, when possible, by using higher
power objectives rather than eyepieces with greater magnifying powers.
Sometimes it is necessary to use a greater magnification than our most
powerful objective will give us; then we must fit our most powerful
eyepiece and draw out the upper part of the microscope tube--in the
best instruments they are made to pull out, after the manner of the
telescope. The effect of so doing will be to increase the magnification
considerably but, at the same time, the definition or clearness is
seriously impaired.
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