Marine biology -- Great Britain; Seashore; Seashore biology
The compound microscope will often prove useful for the examination of
very minute objects, as well as for the study of the structure of the
principal tissues of the larger species; but since detailed instructions
for the management of the microscope, and for the preparation of objects
for microscopic examination would occupy much more space than we can
spare, we shall content ourselves with nothing more than a few general
hints on this portion of the young naturalist’s work, dealing more
particularly with those points which commonly present difficulties to
the amateur.
If it is desired to examine some minute living object, such as a
protozoon, place the object in a drop of the water in which it lived
just in the middle of a clean glass slip, and cover it with a
cover-glass. The quantity of water should be just sufficient to fill the
space between the two glasses. If less than this has been used, a little
more applied to the edge of the cover by means of a glass rod will
immediately run in between the glasses; while if an excessive amount was
employed, the surplus may be removed by the application of a strip of
blotting paper. Place the glass slip on the stage of the microscope, and
reflect light through it from the mirror below.
Examine it first with a low power; and, after having observed as much as
possible of the creature’s movements and structure with this aid, repeat
with a higher power. This rule applies not only to such small objects as
we have now under consideration, but to all objects, and parts of them,
in which minute details are to be observed.
Beginners with the microscope often find prolonged examination very
tiring to the eyes, but this, we believe, would seldom be the case if
right methods were followed. Both eyes should always be open, and the
microscopist should train himself to use both eyes equally for the
actual observation.
The higher the magnifying power used, the nearer must the objective (the
lower combination of lenses) be brought to the object itself, and it is
no uncommon thing for the amateur, in his attempts to focus his object,
to lower the body of the microscope beyond its proper position, causing
the objective to crush the object, break the thin cover-glass, and
become wetted with the liquid, if any, in which the object was being
examined. All this may be avoided by lowering the body of the microscope
until it nearly touches the cover-glass before attempting to view the
object through it, and then, with the eye above the object-glass, to
gradually raise the body until the object is in focus.
[Illustration: FIG. 47.--CELL FOR SMALL LIVING OBJECTS]
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account