Marine biology -- Great Britain; Seashore; Seashore biology
The top of the cover-glass should always be perfectly dry; and if by any
chance the objective becomes wet it should be wiped perfectly dry with a
piece of old silk or with chamois leather. Also, if permanent mounting
is attempted, and the preservative liquid is allowed to come in contact
with the objective, such liquid must, of course, be washed off with some
suitable solvent before any attempt is made to wipe the lens dry.
If the object under examination is of such dimensions that the
cover-glass has a tendency to rock on it, or if it is a living object of
such a size that it is unable to move freely in the exceedingly thin
film of water between the cover and the slip, it should be placed in a
cell. The cell may be made by cementing a ring of glass or vulcanite to
the middle of a slip, or it may be a little circular cavity prepared in
the slip itself. In either case the cell must be quite full of water
before the cover-glass is applied, so that no air-bubbles are included.
Hitherto we have spoken only of mounting small objects in water, and
this is advisable when the object is moist, whether it be animal or
vegetable, alive or dead. But dry objects may be examined in the dry
state, in which case they need not be covered. If they are composed of
transparent material they are to be dealt with in the manner recommended
before, as far as the management of the light is considered; that is, a
moderately strong light is sent through them by the reflector below the
stage; but opaque objects are best examined on a dead black ground, the
light being directed on to them by means of a condensing lens placed
between them and the source of light.
A collector who has done only a few days’ work on the sea shore will
probably find himself the possessor of a host of interesting objects
that will afford much pleasure and instruction when placed under the
microscope--objects, many of which have been somewhat hastily deposited
in a bottle of spirit or other preservative for study in his future
leisure moments. These objects, if small, may be examined as above
described, simply placing them under a cover-glass, or in a cell, with a
clear drop of the same liquid in which they have been kept.
The general characters of the larger objects may also be observed by
means of some kind of hand lens, but even these are generally best
examined under water or other suitable liquid.
A great deal may be learnt of natural objects by preparing very thin
sections for microscopic examination; and although special works should
be consulted if one desires to become proficient in the different
methods of cutting and preparing such sections, yet a great amount of
good work may be done with the aid of a sharp razor, manipulated with
nothing more than ordinary skill.
Public-domain text, read in full here on John Shaqi.
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