The entire contents of the tube are placed in a small mortar and crushed with the pestle. Then 2 to 3 c.c. salt solution are added and the whole rubbed into a uniform mixture or emulsion, after which it is poured into a graduated cylinder holding 50 c.c. A little more salt solution is then added to the mortar, and thus the remaining particles of virus are added to the previous mixture. The fluid in the graduate is then made up to 30 c.c. with salt solution, and then poured into a sterile wide-mouthed bottle holding 100 c.c. Any remaining emulsion is then washed out of the graduate with an additional 10 c.c. salt solution and added to the 30 c.c. in the bottle. The bottle thus contains virus ready to inoculate, and each 2 c.c. will be the dose for the first inoculation of a calf, provided that the tube originally contained 20 I. E. The dose for a second inoculation would then be contained in 10 c.c. of this fluid. VII. INSTRUMENT CASE.--In order to carry out the inoculations in agricultural practice, the instrument case constructed by W. Holzhauer, Marburg, is to be recommended. It contains the following: (_a_) Two bottles for the virus, each 100 c.c., made of colored glass. (_b_) One bottle lysol. (_c_) One bottle alcohol. (_d_) One Erlenmeyer flask (for 1% salt solution). (_e_) One mortar and pestle. (_f_) One graduated cylinder 50 c.c. (_g_) One graduated cylinder of 10 c.c. (_h_) One basin to hold disinfectant solutions. (_i_) Six thermometers with tapes and clamps. (Price 10.50 marks or 1.75 marks each.) (_k_) Two virus syringes with two strong canulas. (Price 11 marks.) (_l_) One small hand-scale and weights. (_m_) One alcohol stove and cover. (_n_) Sterile cotton. (_o_) One wire basket. The entire box is supplied with a canvas cover and a leather handle. The lid, which bears four movable legs, can, when the same is removed from the box, be set up like a saucepan and used as a sterilizer. For this purpose the vessels and instruments are placed into the wire basket, the sterilizer half-filled with 2% lysol solution, and the whole heated just to boiling by means of the alcohol stove. The price of the complete outfit is 85 marks. By omitting the thermometers and syringes the price is reduced to 63.50 marks.