Produced by Juliet Sutherland, Richard Prairie, Charles Franks and the Online Distributed Proofreading Team. THE VITAMINE MANUAL A Presentation of Essential Data About the New Food Factors BY WALTER H. EDDY ASSOCIATE PROFESSOR PHYSIOLOGICAL CHEMISTRY _Teachers College, Columbia University_ CONTENTS CHAPTER I HOW VITAMINES WERE DISCOVERED CHAPTER II THE ATTEMPTS TO DETERMINE THE CHEMICAL NATURE OF A VITAMINE CHAPTER III THE METHODS USED IN TESTING FOR VITAMINES CHAPTER IV THE YEAST TEST FOR VITAMINE B CHAPTER V THE SOURCES OF THE VITAMINES CHAPTER VI THE CHEMICAL AND PHYSIOLOGICAL PROPERTIES OF THE VITAMINES CHAPTER VII HOW TO UTILIZE THE VITAMINES IN DIETS CHAPTER VIII AVITAMINOSES OR THE DISEASES THAT RESULT FROM VITAMINE DEFICIENCIES CHAPTER IX BIBLIOGRAPHY PREFACE The presentation of essential data concerning vitamines to succeeding groups of students has become increasingly difficult with the development of research in this field. The literature itself has assumed a bulk that precludes sending the student to original sources except in those instances when they are themselves to become investigators. The demand on the part of the layman for concise information about the new food factors is increasing and worthy of attention. For all of these reasons it has seemed worth while to collate the existing data and put it in a form which would be available for both student and layman. Such is the purpose of this little book. It has been called a manual since the arrangement aims to provide the student with working material and suggestions for investigation as well as information. The bibliography, the data in the chapter on vitamine testing, the tables and the subdivision of subject matter have all been arranged to aid the laboratory workers and it is the hope that this plan may make the manual of especial value to the student investigator. The management also separates the details necessary to laboratory investigation from the more purely historical aspects of the subject which we believe will be appreciated by the lay reader as well as the student. No apologies are made for data which on publication shall be found obsolete. The whole subject is in too active a state of investigation to permit of more than a record of events and their apparent bearing. Whenever there is controversy the aim has been to cite opposing views and indicate their apparent value but with full realization that this value may be profoundly altered by new data.