In the formation a second time of a cellular floor for the segmentation cavity in the third stage, the Elasmobranch embryo seems to resemble that of the Osseous Fish[135]. Upon this feature great stress is laid both by Dr Götte[136] and Prof. Haeckel[137]: but I am unable to agree with the interpretation of it offered by them. Both Dr Götte and Prof. Haeckel regard the formation of this floor as part of an involution to which the lower layer cells owe their origin, and consider the involution an equivalent to the alimentary involution of Batrachians, Amphioxus, &c. To this question I hope to return, but it may be pointed out that my observations prove that this view can only be true in a very modified sense; since the invagination by which hypoblast and alimentary canal are formed in Amphioxus is represented in Elasmobranchii by a structure quite separate from the ingrowth of cells to form the floor of the segmentation cavity. Footnote 135: This floor appears in most Osseous Fish to be only partially formed. Vide Götte, _loc. cit._ Footnote 136: _Loc. cit._ Footnote 137: _Loc. cit._ The eventual _obliteration_ of the segmentation cavity by cells derived from the yolk is to be regarded as an inherited remnant of the involution by which this obliteration was primitively effected. The passage upwards of cells from the yolk, may possibly be a real survival of the tendency of the hypoblast cells to grow inwards during the process of involution. The last feature of the segmentation cavity which deserves notice is its excentric position. It is from the first situated in much closer proximity to the non-embryonic than to the embryonic end of the blastoderm. This peculiarity in position is also characteristic of the segmentation cavity of Osseous Fishes, as is shewn by the concordant observations of Oellacher[138] and Götte[139]. Its meaning becomes at once intelligible by referring to the diagrams in my paper[140] on the Early Stages in the Development of Vertebrates. It in fact arises from the asymmetrical character of the primitive alimentary involution in all anamniotic vertebrates with the exception of Amphioxus. Footnote 138: _Loc. cit._ Footnote 139: _Loc. cit._ Footnote 140: _Loc. cit._ Leaving the segmentation cavity I pass on to the other features of my sections. There is still to be seen a considerable aggregation of cells at the non-embryonic end of the blastoderm. The position of this, and its relations with the portion of the blastoderm which at an earlier period contained the segmentation cavity, indicate that the growth of the blastoderm is not confined to its edge, but that it proceeds at all points causing the peripheral parts to glide over the yolk. The main features of the cells of this blastoderm are the same as they were in the one last described. In the non-embryonic region the epiblast has thinned out, and is composed of a single row of cells, which, in the succeeding stages, become much flattened.