The lower layer cells over the greater part of their extent, have not undergone any histological changes of importance. Amongst them may frequently be seen a few exceptionally large cells, which without doubt have been derived directly from the yolk. The embryonic rim is now a far more considerable structure than it was. Vide Pl. 7, fig. 7. Its elongation is mainly effected by the continuous conversion of rounded lower layer cells into columnar hypoblast cells at its central or anterior extremity. This conversion of the lower layer cells into hypoblast cells is still easy to follow, and in every section cells intermediate between the two are to be seen. The nature of the changes which are taking place requires for its elucidation transverse as well as longitudinal sections. Transverse sections of a slightly older embryo than B are represented on Pl. 7, fig. 8_a_, 8_b_ and 8_c_. Of these sections _a_ is the most peripheral or posterior, and _c_ the most central or anterior. By a combination of transverse and longitudinal sections, and by an inspection of a surface view, it is rendered clear that, though the embryonic rim is a far more considerable structure in the region of the embryo than elsewhere (compare fig. 6 and fig. 7 and 7_a_), yet that this gain in size is not produced by an outgrowth of the embryo beyond the rest of the germ, but by the conversion of the lower layer cells into hypoblast having been carried far further towards the centre of the germ in the axial line than in the lateral regions of the rim. The most anterior of the series of transverse sections (Pl. 7, fig. 8_c_) I have represented, is especially instructive with reference to this point. Though the embryonic rim is cut through at the sides of the section, yet in these parts the rim consists of hardly more than a continuity between epiblast and lower layer cells, and the lower layer cells shew no trace of a division into mesoblast and hypoblast. In the axis of the embryo, however, the columnar hypoblast is quite distinct; and on it a small cap of mesoblast is seen on each side of the medullary groove. Had the embryonic rim resulted from a projecting growth of the blastoderm, such a condition could not have existed. It might have been possible to find the hypoblast formed at the sides of the section and not at the centre; but the reverse, as in these sections, could not have occurred. Indeed it is scarcely necessary to have recourse to sections to prove that the growth of the embryonic rim is towards the centre of the blastoderm. The inspection of a surface view of a blastoderm at this period demonstrates it beyond a doubt (Pl. 8, fig. B). The embryo, close to which the embryonic rim is alone largely developed, does not project outwards beyond the edge of the germ, but inwards towards its centre.