Then let the ligature be brought to that state of middling tightness which is used in bleeding, and it will be seen that the hand and arm will instantly become deeply suffused and extended, and the veins show themselves tumid and knotted. Which is as much as to say that when the arteries pulsate the blood is flowing through them, but where they do not pulsate they cease from transmitting anything. The veins again being compressed, nothing can flow through them; the certain indication of which is that below the ligature they are much more tumid than above it. Whence is this blood? It must needs arrive by the arteries. For that it cannot flow in by the veins appears from the fact that the blood cannot be forced towards the heart unless the ligature be removed. Further, when we see the veins below the ligature instantly swell up and become gorged when from extreme tightness it is somewhat relaxed, the arteries meanwhile continuing unaffected, this is an obvious indication that the blood passes from the arteries into the veins, and not from the veins into the arteries, and that there is either an anastomosis of the two orders of vessels, or pores in the flesh and solid parts generally that are permeable to the blood. And now we understand wherefore in phlebotomy we apply our fillet above the part that is punctured, not below it. Did the flow come from above, not from below, the bandage in this case would not only be of no service, but would prove a positive hindrance. And further, if we calculate how many ounces flow through one arm or how many pass in twenty or thirty pulsations under the medium ligature, we shall perceive that a circulation is absolutely necessary, seeing that the quantity cannot be supplied immediately from the ingesta, and is vastly more than can be requisite for the mere nutrition of the parts. And the third point to be confirmed is this. That the veins return this blood to the heart incessantly from all parts and members of the body. This position will be made sufficiently clear from the valves which are found in the cavities of the veins themselves, from the uses of these, and from experiments cognisable by the senses. The celebrated Hieronymus Fabricius, of Aquapendente, first gave representations of the valves in the veins, which consist of raised or loose portions of the inner membranes of these vessels of extreme delicacy and a sigmoid, or semi-lunar shape. Their office is by no means explained when we are told that it is to hinder the blood, by its weight, from flowing into inferior parts; for the edges of the valves in the jugular veins hang downwards, and are so contrived that they prevent the blood from rising upwards.