III. By Ethereal Solution of Ammonia. 3·6 per cent. ‘_Kechan._’—By Mayer’s Solution. A. (leaf and midrib) 2·835 per cent. B. (leaf and midrib) 3·0375 ” ” C. (leaf and midrib) 2·85525 ” ” —————— 2·90925 per cent. average. ‘_Shiraz._’—By Mayer’s Solution. A. (leaf and midrib) 5·8725 per cent. B. (leaf and midrib) 5·7975 ” ” —————— 5·835 per cent. average. ----- Footnote D: Dragendorff, ‘Chemische Werthbestimmung starkwirkender Droguen,’ § 63, p. 52 _et seq._ Footnote E: The ‘Analyst,’ January 1886, p. 16; ‘Chem. Zeit.,’ ix., 1886. ----- “Estimation of Saccharoid Matter; calculated as cane sugar.—The fermentation process was the one adopted, not that we consider it by any means a good one, but because it was the only one practicable. Fehling’s solution was inadmissible, owing to the precipitation of colouring and other matters, and the polariscope gave no indication. The objections to the fermentation process are due to the small amount of alcohol produced in the relatively large bulk of liquid. This renders the solution liable to acetification, and the ultimate distillate obtained is very weak in spirit, making it extremely difficult to obtain the correct specific gravity; the specific gravities obtained were always between ·998 and unity. “We worked as follows:—200 grains of dried tumbeki were exhausted by repeated infusion in boiling water. The filtered liquid when cool was mixed with 100 grains of German yeast and allowed to stand three days in a warm place to ferment. “About one-third was then distilled, the distillate being redistilled and three successive fractions of 500 fluid grains collected, the alcohol in each being estimated; the third portion contained little if any spirit. “It being stated that basic acetate of lead removes saccharoid matter from the kindred plant tobacco; we tried its action on the infusion of tumbeki.