“At the onset it was found impossible to thoroughly wash the bulky precipitate caused by the lead; so, to ensure a definite result, sufficient basic acetate of lead was added to the infusion of 200 grains of tumbeki and the whole made up to 30 fluid ounces with distilled water and well mixed. An aliquot part (20 fluid ounces) was then filtered off, excess of lead removed by sulphuretted hydrogen, the sulphide filtered out, the solution boiled to drive off the sulphuretted hydrogen and the infusion, when cool, was fermented in the usual way. But acetic acid was necessarily present from the decomposition of the lead salt by the sulphuretted hydrogen, and this on distilling would tend to raise the specific gravity. To remedy this, slaked lime, or preferably potassic hydrate, was added before redistilling, but considering that from one to three per cent. of ammoniacal salt is contained in the original tumbeki, it is probable that some might still remain and by the action of the fixed alkali furnish a trace of free ammonia which would lower the specific gravity, and thus apparently raise the percentage of alcohol. As far as we can judge basic acetate of lead does not seem to remove fermentable matter from infusion of tumbeki. ────────┬──────────────────┬────────────────── │ I. │ II. ────────┼─────┬────────────┼─────┬──────────── │ │ Pb │ │ Pb │ │ treatment. │ │ treatment. Ispahan │2·64 │ 2·67 │ — │ 2·35 Hidjaz │3·00 │ 2·8 │ 2·7 │ — Kechan │5·58 │ 5·33 │ — │ — Shiraz │3·48 │ 3·88 │3·23 │ 3·1 ────────┴─────┴────────────┴─────┴──────────── “Ash.—The following bases and acids were uniformly found in the ashes:—Sodium, potassium, lithium, magnesium, calcium, iron, aluminium, silica, chlorine, phosphoric acid, sulphuric acid, carbonic acid. GENERAL TABLE OF RESULTS. ─────────────────────────┬─────────┬─────────┬─────────┬───────── │Ispahan. │Hidjaz. │Kechan. │Shiraz. ─────────────────────────┼─────────┼─────────┼─────────┼───────── Nicotine │ 5·4945 │ 2·046 │ 2·909 │ 5·835 Saccharoid matter │ 2·64 │ 2·85 │ 5·58 │ 3·355 Saccharoid matter after │ │ │ │ Pb treatment │ 2·51 │ 2·80 │ 5·33 │ 3·49 Soluble in water │42·0 │42·3 │39·9 │55·6 Insoluble in water │58·0 │57·7 │60·1 │44·4 Ash │22·0 │28·5 │28·5 │26·15 ─────────────────────────┴─────────┴─────────┴─────────┴───────── “The foregoing work has been carried out in the laboratories of the Pharmaceutical Society.”