Venoms: Venomous Animals and Antivenomous Serum-therapeuticsCalmette, A. (Albert)
Science
Venoms: Venomous Animals and Antivenomous Serum-therapeutics
Calmette, A. (Albert)
Antitoxins; Poisonous snakes; Toxins; Venom
It is precisely this desideratum that J. Morgenroth has succeeded in
realising, by means of hydrochloric acid, which renders it possible
to dissociate the neutral mixture, _toxin_ + _antitoxin_, into its
constituent elements, and then to obtain a _lecithide_.
Experiments show that the quantity of lecithide thus restored
absolutely corresponds to that of the cobra-hæmolysin originally added
to the antitoxin, and that the antitoxin set free is not injured by
the hydrochloric acid, even after twenty-four hours of contact. It is
sufficient to add the quantity of soda or of ammonia necessary for the
neutralisation of the acid, in order to see the antitoxin reappear in
its original strength.
It is therefore possible, by causing hydrochloric acid (in a solution
not stronger than 3 per cent.) to act on a neutral mixture of
cobra-hæmolysin (toxin) and antitoxin, to set the former at liberty
in the form of _lecithide_, to withdraw the latter from the action of
the antitoxin, and to demonstrate its presence, owing to its hæmolytic
properties.
It has been found by Kyes and Sachs that, under the influence of
hydrochloric acid, cobra-hæmolysin becomes resistant to heat to such an
extent that it is not destroyed even by prolonged heating at 100° C.
If to a neutral mixture of toxin + antitoxin we add a small quantity of
hydrochloric acid, and then heat the mixture at 100° C., the antitoxin
being in this case destroyed, we shall recover the whole of the toxin
originally employed.
Therefore, as was shown by me so long ago as 1894, if the mixture of
toxin + antitoxin produces a chemical combination between the two
substances, this combination is unstable, and can be effectively broken
up into these two constituent elements by various influences.
CHAPTER XV.
_TREATMENT OF POISONOUS SNAKE-BITES IN MAN AND ANIMALS._
_OBJECTS OF THE TREATMENT: TECHNIQUE OF ANTIVENOMOUS SERUM-THERAPY._
In all countries the remedies recommended for the bites of poisonous
snakes are innumerable, and native pharmacopœias abound in so-called
infallible recipes.
Pliny himself wrote on this subject as follows:--
“For poisonous bites, it is customary to employ a liniment made of
fresh sheep-droppings, cooked in wine. Rats cut in two are also
applied; these animals possess important properties, especially at the
epoch of the ascension of the stars, seeing that the number of a rat’s
fibres wax and wane with the moon.
“Of all birds, those that afford most assistance against snakes are
vultures. The black ones are the weaker. The odour of their feathers
when burnt puts snakes to flight. Provided with a vulture’s heart one
need not fear encounters with snakes, and can also defy the wrath of
wild beasts, robbers, and princes.
Public-domain text, read in full here on John Shaqi.
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