Amebas with so-called limax-shaped bodies do not possess surface layers
that carry particles forward with the same speed as those amebas with
broad bodies. It is only occasionally that large amebas such as
_proteus_ are found in a limax or clavate shape. One of the most
favorable of the large amebas in this respect is _discoides_. It is
frequently found in clavate shape and it possesses the further advantage
in being nearly cylindrical in cross section. It is also more in the
habit of loping along the surface in the manner described by Dellinger
(’06, p. 57) so that what is observed to take place in _discoides_ in
the clavate shape, holds likewise for free pseudopods extended into the
water out of contact with a solid support (Figure 22).
[Illustration: Figure 21. Illustrating the similarity of the movement of
the surface layer of _Amoeba verrucosa_ with that of _A.
sphaeronucleosus_. A group of three particles, connected by dotted lines
for reference, change their relative positions as the ameba
(_verrucosa_) changes its direction of movement. Length of the ameba,
150 microns.]
[Illustration: Figure 22. Illustrating the movements of an _Amoeba
proteus_, after Dellinger. At _c_ in stage 2 a pseudopod is projected
which fastens itself to the substratum as shown at _c_, 3, while _a_, 2,
is pulled loose. In 4 another pseudopod is projected which fastens
itself at _d_. The ameba is not in contact with the substratum at all
points on its under side.]
In figure 23 is shown a clavate _discoides_ with a small particle
attached to its side. The particle moved forward until it came to lie at
the anterior edge, 10. The speed of the particle from 1 to 10 was 1.36
times as fast as that of the ameba, a much slower rate than was observed
in _sphaeronucleosus_. At 6 a new pseudopod was projected for a short
distance, thus increasing the amount of new ectoplasm forming in
proportion to that of the whole ameba. This change was reflected in the
increased speed of the particle, which moved 1.64 times as fast as the
ameba from 5 to 6. At 10 the anterior end again spread out and again
the particle moved faster--twice as fast as the ameba from 9 to 10.
Stages 11, 12, 13 are added to show that the particles do not tend to go
to the under surface but remain at or very near the tip. The slight
irregularity of the waves of hyaloplasm pushed out at the anterior end
accounts for the changing position of the particle after it has reached
the anterior edge. The particle remained at the edge of the advancing
ameba for several minutes after the stage drawn at 13.
[Illustration: Figure 23. Showing the movement of a particle on the
surface layer of an _Amoeba discoides_. The particle remained on the
anterior end of the ameba for several minutes after stage 13. The ameba
was about 320 microns long.]
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