Being desirous to repeat the above experiments on the heads of other oxen, and on those of sheep and lambs, varying the pile, both in regard to its nature and the number of pieces, I constructed three piles of twenty-five, fifty, and a hundred and twenty pieces of silver and zinc. The results, however, differed from the preceding only in the greater or less intensity of the contractions, according as one or the other apparatus was applied to the same animals. I remarked in particular, that the combination most favourable to muscular contractions is obtained, when the arc is established from the ears to the spinal marrow. In this case the eye is so much affected, that the eye-lids open entirely while the eye-ball turns round, and projects somewhat from its socket, as sometimes happens in the most violent madness. EXPERIMENT V. Having provided an ox recently killed, the head of which was not cut off, I formed an arc from one ear to the other, interposing the pile. The immediate result was a commotion so violent in all the extremities of the animal, that several of the spectators were much alarmed, and thought it prudent to retire to some distance. I then cut off the head, and formed an arc from the spinal marrow, first to the diaphragm, and then to the sphincter ani. In the first case, the diaphragm experienced violent contractions; in the other I obtained a very strong action on the rectum, which even produced an expulsion of the fæces. EXPERIMENT VI. To give more extent to my experiments, I thought proper to repeat them on lambs, chickens, and other warm-blooded animals; and without enumerating such phænomena as are common, I shall only observe, that the tongue, which was projected beyond the lips, again returned into the cavity of the mouth, after several applications of the arc, as was the case in the second experiment. The movements of the ears and eye-lids were stronger than in the other parts. Comparative anatomy must explain why this phænomenon, so striking in animals of this kind, is not observed in man. EXPERIMENT VII. The observations which I had made on the Galvanism of the pile excited my curiosity so much, that I was induced to try some comparative experiments by means of common electricity. With this view I placed an iron wire in each ear of a lamb, and discharged through it twice in succession a Leyden flask, the two coatings of which were in communication with the wires applied to the ears. By these means I obtained contractions, but weaker than those produced by the pile; and I always observed the same result in other warm-blooded animals. EXPERIMENT VIII.