Having sawn open the skull, I directed the action of the pile to different parts of the brain, in the same order as they occurred in the course of anatomical dissection. All these parts appeared to be affected by the Galvanic force; but its action was stronger on the corpus callosum and the cerebellum. The same result nearly was obtained, when I repeated the experiment on the heads of different calves and lambs. EXPERIMENT XIII. The heart of an ox, removed from the body, being exposed to the action of Galvanism, though the pile was very powerful, exhibited no signs of muscular contraction. I repeated the same experiment on the heart of an ox, without removing it from the body, and on the hearts of several dogs, one arc being applied to the spinal marrow, while the other touched sometimes the surface of the heart, and sometimes penetrated into its substance; but with the same result: no muscular convulsions were produced. EXPERIMENT XIV. I prepared some frogs; and having waited till the motion of the ventricles of the heart had become very slow, and almost imperceptible, I communicated to them the Galvanic influence, and it appeared to me that some movements were produced in the ventricles. I repeated this experiment lately on the heart of a rabbit, and with the same success. Having tried the hearts of several calves and dogs, I could not observe any decided motion in the ventricles; but I remarked that the Galvanic power exercised a strong action on the auricles. EXPERIMENT XV. Without taking into consideration the differences in the action of Galvanism on the heart, according to the different applications and the different kinds of animals subjected to experiment, I observed, that after this muscle has lost its susceptibility to the action of Galvanism, the other muscles still retain it in a very high degree. This effect is very striking in regard to the heart and the muscles of oxen and dogs; and this corresponds with what has been stated by the Commissioners of the French National Institute in their Report. Speaking of the anomalies found in this respect in the heart, they conclude _that it is at any rate certain that this organ loses, in a very short time, and much sooner than the other muscles, the faculty of being agitated by Galvanism_. EXPERIMENT XVI. All the observations I was able to make on the involuntary muscles will be found in the same Report, from which the following is an extract: “Dr. Grapengiesser says, that he saw the vermicular motion of the intestines increased by the action of Galvanism in a living subject, whose large intestines protruded beyond the abdomen, in consequence of a scrotal hernia. Professor Aldini made us observe the same effect on the intestinal canal of a dog. We perceived also very evident contractions in a portion of the stomach, separated from the animal. We saw the auricles of the heart contract; but never found this to be the case with the ventricles.” EXPERIMENT XVII.