An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
_Dry transparent objects_ are usually small and delicate; for, unless
they are so, their structure cannot be well seen. In mounting these, a
square piece of note-paper or tracing-paper, with the centre cut out,
may be fastened to a clean slide with a little paste, gum, or shellac
varnish. When this is thoroughly dry, the object is placed in the vacant
space, a clean dry cover laid on, and the varnish applied by means of a
hair-pencil to the edges in very small quantities. This will run in
between the under surface of the edges of the cover and the upper
surface of the paper, and when dry will cement the two together.
Supposing that the object is so delicate that it cannot be removed from
the surface of a slide, if it will not be injured by heat, a good plan
is to draw a square or circle around the object with a little black
japan, then to heat the slide gradually until the japan is not indented
with the finger-nail when cold. A clean slide is then laid upon the ring
of japan, the whole again gently warmed, until the varnish is softened,
and the cover lightly pressed so as to be in contact all round with the
varnish. The slide must then be rapidly cooled, by being laid upon a
piece of metal, which prevents the varnish from running in so as to
spoil the object.
Many dry transparent objects can be preserved by mounting in Canada
balsam. This is the best process for mounting objects in general; but
only those can be so preserved which are not injured by drying, and
which are not rendered too transparent by the balsam. If the object to
be mounted in balsam be small, it is thoroughly dried, and then a drop
of oil of turpentine added to it upon a slide; the slide is then gently
warmed, which causes the turpentine to evaporate. When this has nearly
all evaporated, a drop of balsam is allowed to fall upon the object from
the end of a wire held at a distance above the flame of a spirit-lamp. A
warmed cover is next laid upon the balsam, and gentle pressure applied
until the cover is sufficiently depressed. The slide is then kept at a
gentle heat until the balsam is quite hard when cold. The superfluous
portions may be removed with the point of a knife, and any residues
cleaned off with turpentine or a little benzole on a cloth.
Another way consists in laying a cover upon the dry object on a slide,
adding a drop of turpentine, and warming the whole over a spirit-lamp
until all air-bubbles are displaced, then continuing the application of
the heat until most of the turpentine has evaporated. A drop or more of
the balsam may next be applied to the edge of the cover, when it will
run in and mix with the turpentine. The whole is then gently heated
until the balsam is hard when cold, more balsam being added if
necessary, to replace the turpentine which has evaporated.
Public-domain text, read in full here on John Shaqi.
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