An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc. — John Shaqi
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
Hence dry opake objects are usually mounted in such manner as to be
enclosed in a cell, the sides being formed by a ring of glass-tube or
cork, or a square piece of leather, cardboard, or paper, with a hole cut
or punched out of the middle. The glass rings are best; but as they are
expensive, some of the other substances are generally used. The size
and thickness of the material from which the rings are made must
obviously vary according to the size and depth of the object. The rings
are cemented to the middle of ordinary slides; and it is best to keep a
number of them ready prepared. The cementing material must vary
according to the nature of the ring used. If this consists of glass,
Canada-balsam or marine glue is best. In using the former, the ring is
gently heated over the flame of the spirit-lamp, and a thin layer of the
balsam applied to its upper or under surface, by means of an iron wire
with a little balsam on its end; it is next warmed over the spirit-lamp,
so that the surface is entirely and evenly coated. A clean slide is then
slightly heated, the ring laid upon it, and gentle pressure is used to
squeeze out the excess of balsam; and the slide is kept at a gentle
heat, until on cooling the balsam becomes so hard as not to be indented
with the finger-nail. Marine glue is applied in the same way as the
balsam, except that prolonged heat is not required to harden it, for it
becomes hard on cooling. The balsam may also be replaced by black japan
or asphalte.
The pieces of cork, leather, or paper are best fastened to the slides
with solution of shellac or sealing-wax in methylated alcohol, or with
white hard varnish.
When the ring or piece has been firmly fixed to the slide by either of
the above cementing materials, so as to form the sides of the cell, the
bottom is to be covered with a piece of black paper, cut to fit it
exactly, and fastened to the surface of the slide with a little gum, or
of either of the above varnishes. As soon as this is thoroughly dry, the
upper surface of the cell-wall, whether of glass or cork, &c., is thinly
covered with varnish, and a clean thin-glass cover laid upon it, and
very lightly pressed; the object is then permanently preserved.
The main points to be observed are, that the object and varnish are
completely dry, and that the cell is thoroughly closed. If the latter be
not the case, more varnish must be applied to any little openings which
may have been left; and it is better to apply the varnish in very small
quantities at a time, the application being renewed as soon as the
previous layer is quite dry.
Public-domain text, read in full here on John Shaqi.
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