An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc. — John Shaqi
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
When the objects are tolerably flat, and sufficiently firm to bear the
pressure of the cover, they may be mounted by adding a small quantity of
glycerine to them lying on a slide; the cover is then applied, and a
little of the cement mentioned below applied warm with a hair-pencil
around the edges of the cover to fasten it to the slide. Care is
required that the glycerine applied be no more than sufficient; for
wherever it has touched the cover or the slide, the cement will not
adhere. Superfluous portions may be sucked up with a piece of clean
moist sponge or a corner of blotting-paper.
When it is required to mount a large number of objects in a short time,
the cement need only be applied to two opposite sides of the cover,
leaving the other two sides open.
When the objects require to be protected from the pressure of the cover,
the sides of a cell must be made with the cement or black japan upon the
slide before the cover is applied, a further quantity being used to
close the cell as usual.
A very strong solution of chloride of calcium may be used for the same
purposes and in the same way as the glycerine. It has the advantage of
not making the object so transparent; but it has the disadvantage of
crystallizing slightly in a dry atmosphere. In most cases, I prefer it
to glycerine.
A large number of interesting objects cannot, however, be preserved in
either glycerine or chloride of calcium, without their value being
impaired by the cause mentioned above. Many kinds of liquid have been
recommended for preserving these, all agreeing mainly in being
inefficient. The objection to them is, that they are evaporable; and
after the object has been mounted for some time, the liquid creeps
between the cement and the slide or cover, at some spot, and evaporates;
and if the cement be not quite hard, the inner and more liquid portion
of it runs into the cell, and spoils the object. A solution, containing
a grain of salt and a grain of alum to the ounce of distilled water, is
as good as any other; or simply distilled water in which a piece of
camphor has been kept. In use, the cell is first formed by making a
circle or outline square on the slide with black japan, and heating this
carefully until it becomes solid when cold. The object is then laid in
the cell, the liquid added, and the cover applied, any excess being
removed with blotting-paper. The cell is to be closed with old black
japan or gold-size, applied round the margins of the cover with a hair
pencil. A second and a third layer of the varnish may be applied upon
the first, when it has become hard outside. Although black japan and
gold-size are generally used for the cement, I prefer that mentioned
below.
Public-domain text, read in full here on John Shaqi.
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