An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
When large preparations are mounted in liquid, the cell-walls are either
formed of glass rings, or they are built up with four oblong pieces of
glass, cemented to the slide and to each other with marine glue. A very
good preservative liquid for large specimens is a solution of chloride
of zinc, in the proportion of 20 grains to the ounce of distilled water.
A mixture of spirit of wine and water, in the proportion of 1 part to 2,
or 1 to 4, is often used for the same purpose.
When preparations are mounted, the cement and the adjacent parts of the
slide and cover should be coated with a solution of sealing-wax in
spirit, which hardens the exterior of the cement.
The cement above alluded to is made by melting together 5 parts of
rosin, 2 parts of balsam, 1 part of bees’-wax, and 1 of red ochre. The
cement is best kept in a little metallic cup, and melted over a
spirit-lamp when used. It should be applied while hot, with a hair
pencil; and cools very quickly.
The preservative liquids should be kept in corked bottles, a hair pencil
being fixed into the under part of the cork; or, what is better, in
stoppered bottles, the stopper being prolonged to a point nearly
reaching the bottom of the bottle.
As soon as the preparations are mounted, they should be labelled, the
labels being kept ready gummed. The balsam should be kept in a capped
bottle, such as is used for holding solutions of gum, with an iron wire
for removing portions as required. By keeping, the balsam becomes
thicker; it may be thinned by the addition of oil of turpentine, and the
application of a gentle heat.
The black japan, &c., may be procured at any oil-shop; the glass rings,
cell-sides, covers, &c., from Mr. Norman, 178 City Road, or of the
microscope-makers.
Mounted objects should be kept in shallow drawers, and be laid flat--not
standing on edge.
_Magnifying power._--Before entering upon the consideration of the
objects themselves, a word or two must be said upon the magnifying
powers. In the plates of this work, the serial number of each figure is
expressed by large numerals placed above the objects, while the number
of times the object is magnified is indicated by small numerals placed
beneath. The latter must be understood to express the number of times
the drawing is larger than the object in one dimension. Thus,
considering fig. 13, Plate I. to be an inch in width (for it is really
somewhat less), being magnified 150 times in the direction of the width,
the object itself is about 1/150th of an inch in size; and it is
represented magnified 150 times linear, or 150 diameters, as it is
called.
A knowledge of the number of times the object is magnified is of the
greatest importance in making use of the drawings; for, without it, the
observer will be unable to apply such a magnifying power of the
microscope as will enable him to see the structural appearances figured
in the drawings.
Public-domain text, read in full here on John Shaqi.
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