An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
In the exogenous process, a portion of the protoplasm protrudes from the
surface of the cell, carrying the cell-wall before it, so as to form a
little bud-like body; this is next cut off at its point of junction with
the parent-cell, and coated, as in the first case, with a new cell-wall,
so as to form a new cell.
_Preparation._--In examining the vegetable elements and tissues, very
thin sections must be made with a razor or thin sharp knife; these are
then to be placed in a little water on a slide. As the structures are
all minute, the distinctness with which they are seen will mainly depend
upon the proper thinness of the sections. When sections of dry stems are
to be examined, the black margins of the air-bubbles contained in the
cells often render the structure indistinct; these must therefore be
displaced by first wetting the tissue with methylated alcohol, and then
adding water to it in a watch-glass or on a slide; or the tissue may be
soaked in warm water for some hours: and this is mostly requisite in
preparing thin sections of dry tissues.
Attention must also be paid to the manner in which the section is made,
or the direction in which the portion of the plant is cut. There are
three important directions which must be distinguished, producing
transverse, longitudinal, and tangential sections. If the cuts be made
across the length of a stem, for instance, the section is called
transverse. If the cuts be made in the direction of the length, through
the centre, the section is longitudinal; and if the cuts are made in a
direction parallel to a line running down the centre of the stem, but
nearer its margin, it is a tangential section. It is scarcely necessary
to mention that an oblique section is intermediate between a transverse
and a longitudinal section.
CHAPTER IV.
VEGETABLE ORGANS.
The vegetable elements and tissues which have been described form,
either separately or by their combination in various ways, the organs of
plants. To these we shall now pass, and consider the structure of the
principal organs of the members of the vegetable kingdom.
_Leaves._--Leaves in their simplest form consist of a single sheet or
layer of parenchymatous cells or cellular tissue, an example of which
may be found in almost any moss (Pl. III. fig. 30). The granules of
chlorophyll will often be very distinctly seen in these cells. The first
addition to this form of leaf is a row or two of prosenchymatous cells
running longitudinally down the middle of the leaf, so as to form a
rudimentary vein or nerve. In other and more highly developed leaves,
the layers of cells are numerous, and traversed by bundles of
wood-cells, vessels, and ducts (fibro-vascular tissue), forming the
veins,--the entire surface being covered with a skin or membrane, called
the epidermis.
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