An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
_Epider´mis_ (ἑπἱ, upon, δἑρμα, skin).--This membrane is composed of one
or more layers of colourless, closely packed cells (Pl. I. figs. 13 &
28), the colour it occasionally exhibits usually arising from some of
the underlying cells of the leaf being seen through it, or remaining
adherent to it when stripped from the leaf. It is easily separated, by
making a cut in a soft leaf, and peeling it off with a fine pair of
forceps, or by soaking a leaf for some time in water and then stripping
it off. It must be remarked that the epidermis covers not only the
leaves, but every part of the plant.
_Hairs._--Arising from the epidermis are the hairs of plants. These are
thread-like or filamentous prolongations of the epidermis beyond the
surface of the leaf (Pl. I. fig. 12), consisting of cells arranged end
to end. They are often branched, sometimes star-shaped (stellate) (fig.
28), and present great varieties in form, as shown in the figures, the
plants from which these were drawn being mentioned in the Description of
the Plates. Sometimes hairs terminate in a little head (Pl. I. figs. 12
_c_, _d_, _e_), the cell or cells composing which secrete a colouring or
a viscid substance; they are then termed glandular. The hairs of plants
are particularly interesting to the microscopic observer, not only on
account of their curious forms, but in connexion with the remarkable
phenomenon of the circulation of the cell-contents, or _rotation_, as it
is called, observable in them. This is difficult to be perceived by any
one unaccustomed to microscopic observation, because the particles by
which the motion of the cell-contents becomes evident are exceedingly
minute; but practice in the use of a high power will overcome this
difficulty. The hairs which exhibit the phenomenon best are those of the
American Spiderwort (_Tradescan´tia Virgin´ica_), which is to be found
in every garden. It may, perhaps be recognized thus:--The plant is about
a foot and a half high; the leaves are sword-shaped and channelled, and
the flowers are purple, in heads, and 1½ inch in diameter. The hairs are
attached to the sides of the stamens, towards the lower part or base.
The stamens should be carefully picked off with forceps, and placed on a
slide in a drop of water; the hairs should then be separated with the
mounted needles, and a cover applied. Under a low power, the hairs are
seen to be beaded or monil´iform (_moníle_, a necklace), and of a fine
purple colour (Pl. I. fig. 22). On applying a high power, as the
¼-inch, the individual cells will come distinctly into view, and the
nucleus will be seen very clearly as a roundish granular mass (Pl. I.
fig. 23 _a_). On carefully examining the cell-contents, delicate lines
will be observed radiating irregularly from the nucleus, some passing to
the top of the cells, while others run towards its base, as in the
figure; and on very close inspection, the portions of protoplasm of
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