The situation was considerably altered by the death of Francis II, in December, 1560. That event was, on the whole, welcome to Elizabeth, for it destroyed the power of the Guises, and Mary Stuart[62] had now to face her Scottish difficulties without French aid. She was not on good terms with her mother-in-law, Catherine de Medici, who now controlled the destinies of France, and it was evident that she must accept the fact of the Scottish Reformation, and enter upon a conflict with the theocratic tendencies of the Church and with the Scottish nobles who were the pensioners of Elizabeth. On the other hand, although Francis II was dead, his widow survived, young, beautiful, charming, and a queen. The dissolution of her first marriage had removed an actual difficulty from the path of the English queen, but, after all, it only meant that she might be able to contract an alliance still more dangerous. As early as December 31st, 1560, Throckmorton warned Elizabeth that she must "have an eye to" the second marriage of Mary Stuart.[63] The Queen of England had a choice of alternatives. She might prosecute the intrigue with the Earl of Arran, capture Mary on her way to Scotland, and boldly adopt the position of the leader of Protestantism. There were, however, many difficulties, ecclesiastical, foreign, and personal, in such a course. Arran was an impossible husband; Knox and the lords of the congregation made good allies but bad subjects; and the inevitable struggle with Spain would be precipitated. The other course was to attempt to win Mary's confidence, and to prevent her from contracting an alliance with the Hapsburgs, which was probably what Elizabeth most feared. This was the alternative finally adopted by the Queen of England; but, very characteristically, she did not immediately abandon the other possibility. On the pretext that Mary refused to confirm the Treaty of Edinburgh, her cousin declined to grant her request for a safe-conduct from France to Scotland, and spoke of the Scottish queen in terms which Mary took the first opportunity of resenting. "The queen, your mistress," she remarked to the English ambassador who brought the refusal, "doth say that I am young and do lack experience. Indeed I confess I am younger than she is, and do want experience; but I have age enough and experience to use myself towards my friends and kinsfolk friendly and uprightly; and I trust my discretion shall not so fail me that my passion shall move me to use other language of her than it becometh of a queen and my next kinswoman."[64]