Bacteria: Especially as they are related to the economy of nature, to industrial processes, and to the public health — John Shaqi
Bacteria: Especially as they are related to the economy of nature, to industrial processes, and to the public healthNewman, George, Sir
Science
Bacteria: Especially as they are related to the economy of nature, to industrial processes, and to the public health
Newman, George, Sir
Bacteria; Bacteriology
The personal view of the writer after some experience of water
examination would favour a standard of "under 500" being a potable
water, if the 500 were of a nature indicating neither sewage pollution
nor disease. Miquel holds that not more than ten different species of
bacteria should be present in a drinking water, and such is a useful
standard. The presence of rapidly liquefying bacteria _associated
with sewage or surface pollution_ would, even though present in fewer
numbers than a standard, condemn a water. Thus it will be seen that it
is impossible to judge alone by the numbers unless they are obviously
enormously high.
[Illustration: WOLFHÜGEL'S COUNTER]
When we are counting colonies upon a Koch's plate, _Wolfhügel's
counter_ may be used. This is a thin plate of glass a size larger than
Koch's plates, and upon it are scratched squares, each square being
divided into nine smaller squares. The Wolfhügel plate is superimposed
upon the Koch's plate, and the colonies counted in one little square or
set of squares and multiplied.
[Illustration: PETRI'S DISH]
By using flat, shallow, circular glass dishes, generally known as
_Petri's dishes_, instead of Koch's plates, much manipulation and time
is saved, and, on the whole, less risk of pollution occurs. Moreover,
these are easily carried about and transferred from place to place.
When counting colonies in a Petri's dish it is sufficient to divide the
circle into eight equal divisions, and counting the colonies in the
average divisions, multiply and reduce to the common denominator of one
cc. For example, if the colonies of the plate appear to be distributed
fairly uniformly we count those in one of the divisions. They reach,
we will suppose, the figure of 60; 60 × 8=480 micro-organisms in the
amount taken from the suspected water and added to the melted gelatine
from which the plate was made. This amount was .25 cc. Therefore we
estimate the number of micro-organisms in the suspected water as 60 ×
8=480 × 4= 1920 m.-o. per cc., which is over standard by about 1500.
A water might then be condemned upon its quantitative examination
alone or qualitative alone, or both. If the quantity were even that
of an artesian well, say 4-10 m.-o. per cc., but those four or ten
were all _Bacillus typhosus_, it would clearly be condemned on its
quality, though quantitatively it was an almost pure water. If, on the
contrary, the water contained 10,000 m.-o. per cc., and none of them
disease-producing, it would still be condemned on the ground that so
large a number of organisms indicated some kind of organic pollution
to supply pabulum for so many organisms to live in one cc. of the
water. It is not the number _per se_ which condemns. The large number
condemns because it indicates probable pollution with surface water or
sewage in order to supply pabulum for so many bacteria per cc.
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