Common objects of the microscopeWood, J. G. (John George)
Science
Common objects of the microscope
Wood, J. G. (John George)
Microscopes; Microscopy
The apparatus required is not necessarily complicated. Reduced to its
elements, it consists essentially only of a razor to cut the sections
and a dish to receive them. It but seldom happens, however, that the
relations of the parts in sufficiently thin sections can be preserved
by such a rough-and-ready method, and frequently the object to be cut
is of such small dimensions as to render it impossible to deal with it
in this way. It is therefore necessary to “imbed” it, so as to obtain
a handle by which to hold it, in such a way that it shall be equally
supported in all directions. Moreover, since the human hand can only
in exceptional cases be brought to such a pitch of skill as to cut a
series of sections, or even single ones, of the needful delicacy, some
mechanical means of raising the object through a definite distance is
highly desirable. The writer has cut many thousands of sections with
the “free hand,” but the personal equation is a large one, and is not
always the same in the same person. For single sections the method
will, with practice, succeed very well, but some means of securing
a number of sections of more or less the same thickness is usually
required.
Let us deal with the imbedding first.
If it be desired to imbed a tissue which has merely been fixed
with formalin, the block should be immersed in strong gum (made by
saturating water with picked gum arabic, white and clean) for several
days. It is then taken out and, without draining, transferred to the
plate of a freezing microtome, and the sections cut from the frozen
block, and mounted in glycerine at once.
This plan is of limited usefulness, since it allows of very little
differentiation of the tissue elements, and that only optical.
Public-domain text, read in full here on John Shaqi.
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