Moray and his friends were thrown by the event into a rebellion; which, however, quickly ended in his defeat and exile. The queen then ruled for some time with the assistance of her husband and of her Italian secretary, David Riccio. In such circumstances, it was unavoidable that the confidence of her Protestant people should abate. Darnley soon proved to be little worth the sacrifice she had made for his sake. By a freak of youthful folly, prompted by jealousy of Riccio’s influence, he associated in a conspiracy with the banished Moray and his associates, for putting the Italian away from the queen; thinking he might then bear undivided sway. Riccio was assassinated at Holyroodhouse, in the queen’s presence (March 9, 1566), and the Protestant lords immediately returned. The horrible outrage took a strong hold of Mary’s feelings, and was allowed too much to sway her subsequent actions. She seemed, however, to be reconciled to her husband; and not long after, her son, who afterwards became James VI., was born (June 19, 1566). The childishness and low habits of Darnley completely unfitted him to become an adviser and help to the queen; he proved, on the contrary, a source of great trouble and vexation. Indignant as she was at Moray, Morton, and other Protestant lords who had been concerned in the Riccio assassination, she was little inclined to lean upon them as before. As the only remaining resource, she began to give her confidence to the Earl of Bothwell and the Earl of Huntly, two nobles of great power, but whose administration could not bring her so much popularity. Bothwell was a man of coarse character, fully as much disposed as any man in that age to gain his ambitious ends by violence. As early as March 1561-2, he had formed a plan for seizing the queen’s person, and carrying her to the castle of Dumbarton, that he and the Duke of Chatelherault might enjoy the government between them. He had since then been restored to favour; but, so far from the queen having ever appeared to regard him as a lover, she had, so lately as February 1565-6, promoted and sanctioned his marriage to a friend of her own, a sister of the Earl of Huntly. He seems now to have thought that an opportunity was presented for his acquiring a mastery in Scotland. He caused the wretched Darnley to be murdered at his lodging in the Kirk of Field, near Edinburgh (March 12, 1567). Being suspected and accused of this act, he submitted to a trial, but was able to overbear justice, and to maintain his place in the queen’s councils. Mary, consequently, suffered in reputation, though whether she was aware of Bothwell’s guilt is to this day a matter of doubt; much less is it certain that she had, as has been suspected, a guilty knowledge of her husband’s death.