Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
The =Greenough binocular microscope=, as shown in Fig. 19, consists
of a microscope stage with two tubes mounted side by side and moving
on the same rack and pinion for the focusing adjustment. Either tube
can be used without the other. The oculars are capable of more or
less separation to suit the eyes of different observers. In each
of the drub-like mountings, near the point where the oculars are
introduced, porro-prisms have been placed, which erect the image.
This microscope gives most perfect stereoscopic images, which are
erect instead of inverted, as in the monocular compound microscopes.
The Greenough binocular microscope is especially adapted for
dissection and for studying objects of considerable thickness.
POLARIZATION MICROSCOPE
The =polarization microscope= (Fig. 20) is used chiefly for the
examination of crystals and mineral sections as well as for the
observation of organic bodies in polarized light. It can, however,
also be used for the examination of regular biological preparations.
[Illustration: FIG. 20.--Polarization Microscope]
If compared with the regular biological microscope, the polarization
microscope is found characteristic of the following points: it is
supplied with a polarization arrangement. The latter consists of a
polarizer and analyzer. The polarizer is situated in a rotating mount
beneath the condensing system. The microscope, of which the diagram
is shown, possesses a triple “Ahrens” prism of calcite. The entering
light is divided into two polarized parts, situated perpendicularly
to each other. The so-called “ordinary” rays are reflected to one
side by total reflection, which takes place on the inner cemented
surface of the triple prism, allowing the so-called “extraordinary”
rays to pass through the condenser. If the prism is adjusted to
its focal point, it is so situated that the vibration plane of the
extra-ordinary rays are in the same position as shown in the diagram
of the illustration.
The analyzer is mounted within the microscope-tube above the
objective. Situated on a sliding plate, it can be shifted into
the optical axis whenever necessary. The analyzer consists of a
polarization prism after Glan-Thompson. The polarization plane of the
active extraordinary rays is situated perpendicularly to the plane as
shown in the diagram. The polarization prisms are ordinarily crossed.
In this position the field of the microscope is darkened as long as
no substance of a double refractive index has been introduced between
the analyzer and polarizer. In rotating the polarizer up to the mark
90, the polarization prisms are mounted parallel and the field of
the microscope is lighted again. Immediately above the analyzer and
attached to the mounting of the analyzer a lens of a comparatively
long focal length has been placed in order to overcome the difference
in focus created by the introduction of the analyzer into the optical
rays.
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