Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
The condensing system is mounted on a slider, and, furthermore,
can be raised and lowered along the optical centre by means of a
rack-and-pinion adjustment. If lowered sufficiently, the condensing
system can be thrown to the side to be removed from the optical
rays. The condenser consists of three lenses. The two upper lenses
are separately mounted to an arm, which permits them to be tilted to
one side in order to be removed from the optical rays. The complete
condenser is used only in connection with high-power objectives.
As far as low-power objectives are concerned, the lower condensing
lens alone is made use of, and the latter is found mounted to the
polarizer sleeve. Below the polarizer and above the lower condensing
lens an iris diaphragm is found.
The microscope table is graduated on its periphery, and, furthermore,
carries a vernier for more exact reading.
The polarization microscope is not furnished with an objective
nose-piece. Every objective, however, is supplied with an individual
centring head, which permits the objective to be attached to
an objective clutch-changer, situated at the lower end of the
microscope-tube. The centring head permits the objectives to be
perfectly centred and to remain centred even if another objective is
introduced into the objective clutch-changer.
At an angle of 45 degrees to the polarization plane of polarizer and
analyzer, a slot has been provided, which serves for the introduction
of compensators.
Between analyzer and ocular, another slot is found which permits
the Amici-Bertrand lens to be introduced into the optical axis. The
slider for the Bertrand lens is supplied with two centring screws
whereby this lens can be perfectly and easily centred. The Bertrand
lens serves the purpose of observing the back focal plane of the
microscope objective. In order to allow the Bertrand lens to be
focused, the tube can be raised and lowered for this purpose. An iris
diaphragm is mounted above the Bertrand lens.
If the Bertrand lens is shifted out of the optical axis, one can
observe the preparation placed upon the microscope stage and,
depending on its thickness or its double refraction, the interference
color of the specimen. This interference figure is called the
orthoscopic image and, accordingly, one speaks of the microscope as
being used as an “orthoscope.”
After the Bertrand lens has been introduced into the optical axis,
the interference figure is visible in the back focal plane of the
objective. Each point of this interference figure corresponds to
a certain direction of the rays of the preparation itself. This
arrangement permits observation of the change of the reflection of
light taking place in the preparation, this in accordance with the
change of the direction of the rays. This interference figure is
called the conoscopic image, and, accordingly, the microscope is used
as a “conoscope.”
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