Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
In making microscopic measurements it is only necessary to find how
many lines of the ocular scale are covered by the object. The number
of lines multiplied by the equivalent of each line will be the size
of the object in microns, or _micromillimeters_.
[Illustration: FIG. 24.--Micrometer Eye-Piece]
MICROMETER EYE-PIECES
=Micrometer eye-pieces= (Figs. 23 and 24) may be used in making
measurements. These eye-pieces with micrometer combinations are
preferred by some workers, but the ocular micrometer will meet the
needs of the average worker.
MECHANICAL STAGES
Moving objects by hand is tiresome and unsatisfactory, first, because
of the possibility of losing sight of the object under observation,
and secondly, because the field cannot be covered so systematically
as when a mechanical stage is used for moving slides.
The =mechanical stage= (Fig. 25) is fastened to the stage by a screw.
The slide is held by two clamps. There is a rack and pinion for
moving the slide to left or right, and another rack and pinion for
moving the slide forward and backward.
[Illustration: FIG. 25. Mechanical Stage]
CAMERA LUCIDA
The =camera lucida= is an optical mechanical device for aiding the
worker in making drawings of microscopic objects. The instrument is
particularly necessary in research work where it is desirable to
reproduce an object in all its details. In fact, all reproductions
illustrating original work should be made by means of the camera
lucida or by microphotography.
[Illustration: FIG. 26.--Camera Lucida]
[Illustration: FIG. 27.--Camera Lucida]
A great many different types of camera lucidas or drawing apparatus
are obtainable, varying from simple-inexpensive to complex-expensive
forms. Figs. 26, 27, and 28 show simple and complex forms.
[Illustration: FIG. 28.--Drawing Apparatus]
MICROPHOTOGRAPHIC APPARATUS
The =microphotographic apparatus= (Fig. 29), as the name implies, is
an apparatus constructed in such a manner that it may be attached to
a microscope when we desire to photograph microscopic objects. It
consists of a metal base and a polished metal pillar for holding the
bellows, slide holder, ground-glass observation plate, and eye-piece.
In making photographs, the small end of the bellows is attached to
the ocular of the microscope, the focus adjusted, and the object or
objects photographed. More uniform results are obtained in making
such photographs if an artificial light of an unvarying candle-power
is used.
[Illustration: FIG. 29.--Microphotographic Apparatus]
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