Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
There are obtainable more elaborate microphotographic apparatus than
the one figured and described, but for most workers this one will
prove highly satisfactory. It is possible, by inclining the tube of
the microscope, to make good microphotographs with an ordinary plate
camera. This is accomplished by removing the lens of the camera and
attaching the bellows to the ocular, focusing, and photographing.
CHAPTER IV
HOW TO USE THE MICROSCOPE
In beginning work with the compound microscope, place the base of the
microscope opposite your right shoulder, if you are right-handed; or
opposite your left shoulder, if you are left-handed. Incline the body
so that the ocular is on a level with your eye, if necessary; but
if not, work with the body of the microscope in an erect position.
In viewing the specimen, keep both eyes open. Use one eye for
observation and the other for sketching. In this way it will not be
necessary to remove the observation eye from the ocular unless it be
to complete the details of a sketch.
=Learn to use both eyes.= Most workers, however, accustom themselves
to using one eye; when they are sketching, they use both eyes,
although it is not necessary to do so.
=Open the iris diaphragm=, and incline the mirror so that white light
is reflected on the Abbé condenser. Place the slide on the centre
of the stage, and if the slide contains a section of a plant, move
the slide so as to place this specimen over the centre of the Abbé
condenser. Then lower the body by means of the coarse adjustment
until the low-power object, which should always be in position when
work is begun, is within one-fourth of an inch of the stage. Then
raise the body by means of the coarse adjustment until the object,
or objects, in case a powder is being examined, is seen. Open and
close the iris diaphragm, finally adjusting the opening so that the
best possible illumination is obtained for bringing out clearly
the structure of the object or objects viewed. Then regulate the
focus by moving the body up or down by turning the fine adjustment.
When studying cross-sections or large particles of powders, it is
sometimes desirable to make low-power sketches of the specimen. In
most cases, however, only sufficient time should be spent in studying
the specimen to give an idea of the size, structure, and general
arrangement or plan or structure if a section of a plant, or, if
a powder, to note its striking characters. All the finer details
of structure are best brought out with the high-power objective in
position.
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