Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
It has been generally supposed that the best possible illumination
for microscopic work is diffused sunlight obtained from a northern
direction. No matter from what direction diffused sunlight is
obtained, it will be found suitable for microscopic work. In no case
should direct sunlight be used, because it will be found blinding
in its effects upon the eyes. Natural illumination--diffused
sunlight--varies so greatly during the different months of the
year, and even during different periods of the day, that individual
workers are resorting more and more to artificial illumination. The
particular advantage of such illumination is due to the fact that
its quality and intensity are uniform at all times. There are many
ways of securing such artificial illumination, no one of which has
any particular advantage over the other. Some workers use an ordinary
gas or electric light with a color screen placed in the sub-stage
below the iris diaphragm. In other cases a globe filled with a weak
solution of copper sulphate is placed in such a way between the
source of light and the microscope that the light is focused on the
mirror. Modern mechanical ingenuity has devised, however, a number of
more convenient micro lamps (Fig. 30). These lamps are a combination
of light and screen. In some forms a number of different screens come
with each lamp, so that it is possible to obtain white-, blue-, or
dark-ground illumination. The type of the screen used will be varied
according to the nature of the object studied.
CARE OF THE MICROSCOPE
If possible, the microscope should be stored in a room of the same
temperature as that in which it is to be used. In any case, avoid
storing in a room that is cooler than the place of use, because when
it is brought into a warmer room, moisture will condense on the
ocular objectives and mirrors.
Before beginning work remove all moisture, dust, etc., from the inner
and outer lenses of the ocular, the objectives, the Abbé condenser,
and the mirror by means of a piece of soft, old linen. When the work
is finished the optical parts should be thoroughly cleaned.
If reagents have been used, be sure that none has got on the
objectives or the Abbé condenser. If any reagent has got on these
parts, wash it off with water, and then dry them thoroughly with soft
linen.
The inner lenses of the eye-pieces and the under lens of the Abbé
condenser should occasionally be cleaned. The mechanical parts of the
stand should be cleaned if dust accumulates, and the movable surfaces
should be oiled occasionally. Never attempt to make new combinations
of the ocular or objective lenses, or transfer the objectives or
ocular from one microscope to another, because the lenses of any
given microscope form a perfect lens system, and this would not be
the case if they were transferred. Keep clean cloths in a dust-proof
box. Under no circumstances touch any of the optical parts with your
fingers.
Public-domain text, read in full here on John Shaqi.
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