Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
PREPARATION OF SPECIMENS FOR CUTTING
Most drug plants are supplied to pharmacists in a dried condition.
It is necessary, therefore, to boil the drug in water, the time
varying from a few minutes, in the case of thin leaves and herbs, up
to a half hour if the drug is a thick root or woody stem. If a green
(undried) drug is under examination, this first step is not necessary.
If the specimen to be cut is a leaf, a flower-petal, or other thin,
flexible part of a plant, it may be placed between pieces of elder
pith or slices of carrot or potato before cutting.
SHORT PARAFFIN PROCESS
In most cases, however, more perfect sections will be obtained if the
specimens are embedded in paraffin, by the quick paraffin process,
which is easily carried out.
After boiling the specimen in water, remove the excess of moisture
from the outer surface with filter paper or wait until the water has
evaporated. Next make a mould of stiff cardboard and pour melted
paraffin (melting at 50 or 60 degrees) into the mould to a height of
about one-half inch, when the paraffin has solidified. This may be
hastened by floating it on cool or iced water instead of allowing it
to cool at room temperature.
The specimens to be cut are now placed on the paraffin, with glue, if
necessary, to hold them in position, and melted paraffin poured over
the specimens until they are covered to a depth of about one-fourth
of an inch. Cool on iced water, trim off the outer paraffin to the
desired depth, and the Specimen will be in a condition suitable for
cutting.
Good workable sections may be cut from specimens embedded by this
quick paraffin method. After a little practice the entire process
can be carried out in less than an hour. This method of preparing
specimens for cutting will meet every need of the pharmacognosist.
LONG PARAFFIN PROCESS
In order to bring out the structure of the =protoplast= (living part
of the cell), it will be necessary to begin with the living part of
the plant and to use the long paraffin method or the collodion method.
Small fragments of a leaf, stem, or root-tip are placed in
chromic-acid solution, acetic alcohol, picric acid, chromacetic
acid, alcohol, etc., depending upon the nature of the specimen under
observation. The object of placing the living specimen in such
solutions is to kill the protoplast suddenly so that the parts of the
cell will bear the same relationship to each other that they did in
the living plant, and to fix the parts so killed.
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