Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
=Oil of Cloves= is used as a clearing fluid for sections preparatory
to mounting in Canada balsam.
=Canada Balsam= is used as a permanent mounting medium for dehydrated
specimens, and as a cement for ringing slides.
=Paraffin= is used for general embedding and infiltrating.
=Lugol’s Solution= is used as a test for starch and for aleurone
grains and proteid matters.
=Osmic Acid=. A two per cent solution is used as a test for fixed
oils.
=Alcohol, Glycerine, and Water Mixture= is used as a temporary
mounting medium and as a qualitative test for fixed oils.
=Chlorzinc Iodide= is used as a test for suberin, lignin, cellulose,
and starch.
=Analine Chloride= is used as a test for lignified cell walls of bast
fibres and of stone cells.
=Phloroglucin=. A one per cent alcoholic solution is used in
connection with hydrochloric acid as a test for lignin.
=Hæmatoxylin-Delifields= is used as a test for cellulose.
[Illustration: FIG. 38.--Reagent Set]
REAGENT SET
Each worker should be provided with a set of =reagent bottles=
(Fig. 38). Such a set may be selected according to the taste of the
individual, but experience has shown that a 30 c.c. bottle with a
ground-in pipette and a rubber bulb is preferable to other types. In
such forms the pipettes are readily cleaned, and the rubber bulbs can
be replaced when they become old and brittle. The entire set should
be protected from dust by keeping it in a case, the cover of which
should be closed when the set is not in use.
MEASURING CYLINDER
In order accurately to measure micro-chemical reagents, it is
necessary to have a standard 50 c.c. cylinder (Fig. 39) graduated to
c.c.’s. Such a cylinder should form a part of the reagent set.
[Illustration: FIG. 39.--Measuring Cylinder]
STAINING DISHES
[Illustration: FIG. 40.--Staining Dish]
There is a great variety of =staining dishes= (Fig. 40), but for
general histological work a glass staining dish with groves for
holding six or more slides and a glass cover is most desirable.
CHAPTER VI
HOW TO MOUNT SPECIMENS
The method of procedure in mounting specimens for study varies
according to the nature of the specimen, its preliminary treatment,
and the character of the mount to be made. As to duration, mounts are
either temporary or permanent.
TEMPORARY MOUNTS
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