Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
the glycerine jelly by first passing the blade of a knife, held in
a vertical position, the back of the knife being next to the slide,
around the edge of the cover glass. After turning the knife-blade so
that the flat side is in contact with slide, remove the jelly outside
of the cover glass. Any remaining fragments should be removed with
a piece of old linen or cotton cloth. Finally, ring the edge of the
cover glass with microscopical cement, of which there are many types
to be had. If the cleaning has been done thoroughly, there is no
better ringing cement than Canada balsam.
In mounting cross-sections, the method of procedure is similar to the
above, with the exception that the glycerine jelly is placed at the
side of the specimen and not in the centre. While melting the jelly,
incline the slide in order to allow the melted glycerine jelly to
flow gradually over the specimen, thus replacing the air contained in
the cells and intercellular spaces. Finish the mounting as directed
above, but under no conditions should you stir the glycerine jelly
with the section.
If specimens, after having been embedded in paraffin or collodion,
are cut, cleared, stained, and dehydrated, they are usually mounted
in Canada balsam. A small drop of this substance, which may be
obtained in collapsible tubes, is placed at one side of the specimen.
While inclining the slide, gently heat until the Canada balsam covers
the specimen. Secure a cover glass by the aid of pliers, pass it
through the flame three times, and lower it slowly while holding it
in an inclined position. Press gently on the cover glass with the
needle-handle, and keep in a horizontal position for twenty-four
hours, then place directly in a slide box or cabinet, since no
sealing is required.
Glycerine is sometimes used to make permanent mounts, but it is
unsatisfactory, because the cover glass is easily removed and the
specimen spoiled or lost, unless ringed--a procedure which is not
easily accomplished. If the specimen is to be mounted in glycerine,
it must first be placed in a mixture of alcohol, glycerine, and
water, and then transferred to glycerine. Lactic acid is another
permanent liquid-mounting medium, which is unsatisfactory in the same
way as glycerine, but like glycerine, there are certain special cases
where it is desirable to use it. When this is used, the slides should
be kept in a horizontal position, unless ringed.
COVER GLASSES
Great care should be used In the selection of =cover glasses=,
however, not only as regards their shape but as to their thickness.
The standard tube length of the different manufacturers makes an
allowance of a definite thickness for cover glasses. It is necessary,
therefore, to use cover glasses made by the manufacturer of the
microscope in use.
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