The Rothamsted experiments supported Asō’s work on the action of manganese sulphate on barley, concentrations of the salt above 1/100,000 having a retarding influence on the growth, the roots being coloured brown and the leaves also showing discolouration. At an early stage in growth the lower leaves of the plants receiving the most poison began to be flecked with brown spots, which were at first attributed to an attack of rust. Suspicion was soon aroused, however, and a closer microscopic investigation showed that no disease was present, but that the cells in the affected spots were dead and brown, though they retained their shape. The dead cells at first occurred in small patches, which spread and coalesced until ultimately the whole leaf was involved. Some of the affected leaves were detached and fused with a mixture of sodium carbonate and potassium nitrate. On dissolving up the resulting mass with water a green colouration was obtained, indicating the presence of manganese in the leaves. This shows that the manganese is taken up by the roots, transferred to the leaves and then deposited in them, the lower leaves being the first affected. The presence of manganese in the nutritive solution retarded the ripening of the grain to some extent, as when the grains from the control plants were hard and ripe, those from plants treated with 1/10,000 MnSO₄ were green, those with 1/100,000 were a mixture of ripe, half-ripe, and green grains, while plants which had received 1/1,000,000 MnSO₄ possessed ripe grains. Peas give similar results to barley so far as the vegetative growth is concerned, the same retardation with the higher concentrations being observed, while the brown discoloured patches in the lower leaves are much in evidence. All traces of manganese in the leaves disappear when the concentration falls to 1/250,000. On the whole peas are more sensitive to manganese poisoning than is barley, and the higher strengths of manganese prove more deleterious to them. (_b_) _Toxic action of manganese compounds in sand cultures._ Little work has been done on this aspect of the problem. Prince de Salm Horstmar (1851) grew oats in sand with various combinations of nitrogenous substances and inorganic mineral salts. He stated that until the time of fruit formation manganese does not seem to be essential to the oat unless iron is in excess in the substratum. (_c_) _Toxic action of manganese compounds in soil cultures._