The next year (1904) the experiments were extended to observe the after effects of the initial doses of manganese sulphate. The harvest of grain was greatest in those plots that had received 30 kgm. Mn₂O₃ per hectare, while it was approached very closely by that from the plot with 25 kgm. Mn₂O₃, which had proved the best in the first year’s experiments. The maximum increase of yield over the unmanured plots in the first year was 37%, while in the second year it dropped to 16·9%. Asō (1904) also obtained an increase of one-third in produce of grain when 25 kgm. Mn₃O₄ per hectare (as manganous chloride) was applied to rice. The development of the plants was improved and the treated plants flowered about four days before the untreated ones. Loew and Honda (1904) grew _Cryptomeria japonica_ in beds, treating the soil with various manures and with iron or manganese sulphate. The latter favoured increase in height, and within 1½ years the cubic content of the trees had increased to double. Fukutome (1904) grew flax in pot cultures, each pot containing 8 kgm. soil, to which was added ·4 gm. MnCl₂.4H₂O and ·4 gm. FeSO₄.7H₂O. This mixture had a marked effect on the growth of the flax, but the individual salts in doses of ·4 gm. per 8 kgm. soil had but little effect. Namba (1908) applied manganese salts to onion plants in pots with a considerable measure of success. Pots containing 8 kgm. loamy soil were manured and received: (1) no manganese, (2) ·1 gm. MnSO₄.4H₂O, (3) ·2 gm. MnSO₄.4H₂O, the manganese sulphate being applied in high dilution as top dressing. The bulbs and leaves were considerably stimulated by small doses of manganese sulphate, the best results being obtained from (2), which represents a manuring of 22 kgm. MnSO₄ per hectare. An increase of the dose lessens the beneficial effect, as the toxic action begins to come into play. The actual figures obtained may prove of interest. Wt. leaves Wt. bulbs Total weight Bulbs & roots & roots Absolute Relative _____________ leaves gm. gm. gm. gm. 1. 29·5 8·5 38·0 100·0 ·28 2. 38·0 22·5 60·5 159·2 ·59 3. 35·5 16·5 51·0 134·2 ·46